Characterization of a molt-inhibiting hormone (MIH) receptor in the Y-organ of the kuruma prawn, Marsupenaeus japonicus

被引：25

作者：

Asazuma, H

Nagata, S

Katayama, H

Ohira, T

Nagasawa, H ^{[1
]}

机构：

[1] Univ Tokyo, Grad Sch Agr & Life Sci, Dept Appl Biol Chem, Bunkyo Ku, Tokyo 1138657, Japan

[2] Japan Int Res Ctr Agr Sci, Tsukuba, Ibaraki 3058686, Japan

来源：

TRENDS IN COMPARATIVE ENDOCRINOLOGY AND NEUROBIOLOGY | 2005年 / 1040卷

关键词：

molt-inhibiting hormone; hormone receptor; Marsupenaeus japonicus;

D O I：

10.1196/annals.1327.027

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

To characterize a receptor for molt-inhibiting hormone (MIH) in the kuruma prawn, Marsupenaeus japonicus, we performed a binding assay and chemical cross-linking experiments using radiolabeled recombinant MIH ([I-125]rMIH). The specific binding of [I-125]rMIH was found in the membrane fraction of the Y-organ (K-d = 4.76 X 10(-10) M and B-max = 5.51 X 10(-12) M). Chemical cross-linking between [1251]rMIH and the membrane fraction of the Y-organ revealed that the MIH receptor protein has a molecular size of approximately 70 kDa.

引用

页码：215 / 218

页数：4

共 4 条

[1] The solution structure of molt-inhibiting hormone from the kuruma prawn Marsupenaeus japonicus [J].