Vaccination With a Latch Peptide Provides Serotype-Independent Protection Against Group B Streptococcus Infection in Mice

被引：17

作者：

Lin, Shun-Mei ^{[1
,2
]}

Jang, A-Yeung ^{[1
,5
]}

Zhi, Yong ^{[1
,6
]}

Gao, Shuang ^{[1
,2
]}

Lim, Sangyong ^{[1
,6
]}

Lim, Jae Hyang ^{[7
]}

Song, Joon Young ^{[8
]}

Sullam, Paul M. ^{[9
,10
,11
]}

Rhee, Joon Haeng ^{[2
,3
,4
]}

Seo, Ho Seong ^{[1
,6
]}

机构：

[1] Korea Atom Energy Res Inst, Div Biotechnol, Jeongeup 56212, South Korea

[2] Chonnam Natl Univ, Sch Med, Dept Mol Med, Brain Korea Program Leading Univ & Students 21, Gwangju, South Korea

[3] Chonnam Natl Univ, Sch Med, Dept Microbiol, Gwangju, South Korea

[4] Chonnam Natl Univ, Sch Med, Clin Vaccine Res & Dev Ctr, Gwangju, South Korea

[5] Chonbuk Natl Univ, Dept Biol Sci, Jeonju, South Korea

[6] Univ Sci & Technol, Dept Radiat Biotechnol & Appl Radioisotope Sci, Daejeon, South Korea

[7] Ewha Womans Univ, Dept Microbiol, Coll Med, Seoul, South Korea

[8] Korea Univ, Dept Internal Med, Coll Med, Seoul, South Korea

[9] Vet Affairs Med Ctr, Div Infect Dis, San Francisco, CA 94121 USA

[10] Univ Calif San Francisco, Dept Med, San Francisco, CA USA

[11] Northern Calif Inst Res & Educ, San Francisco, CA USA

来源：

JOURNAL OF INFECTIOUS DISEASES | 2018年 / 217卷 / 01期

基金：

美国国家卫生研究院; 新加坡国家研究基金会;

关键词：

Streptococcus agalactiae; serine; rich repeat; latch; vaccine; BLOOD-BRAIN-BARRIER; SURFACE-PROTEINS; BINDING; FIBRINOGEN; AGALACTIAE; IDENTIFICATION; ANTIBODIES; DISCOVERY; VACCINES; GORDONII;

D O I：

10.1093/infdis/jix565

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Streptococcus agalactiae (group B streptococcus [GBS]) is a leading cause of invasive diseases in neonates and severe infections in elderly individuals. GBS serine-rich repeat glycoprotein 1 (Srr1) acts as a critical virulence factor by facilitating GBS invasion into the central nervous system through interaction with the fibrinogen A alpha chain. This study revealed that srr1 is highly conserved, with 86.7% of GBS clinical isolates expressing the protein. Vaccination of mice with different Srr1 truncated peptides revealed that only Srr1 truncates containing the latch domain protected against GBS meningitis. Furthermore, the latch peptide alone was immunogenic and elicited protective antibodies, which efficiently enhanced antibody-mediated opsonophagocytic killing of GBS by HL60 cells and provided heterogeneous protection against 4 different GBS serogroups. Taken together, these findings indicated that the latch domain of Srr1 may constitute an effective peptide vaccine candidate for GBS.

引用

页码：93 / 102

页数：10

共 37 条

[1] A Safe and Stable Neonatal Vaccine Targeting GAPDH Confers Protection against Group B Streptococcus Infections in Adult Susceptible Mice [J].