Development of colloidal gold immunochromatographic signal-amplifying system for ultrasensitive detection of Escherichia coli O157:H7 in milk

被引：57

作者：

Wang, Jing-Yun ^{[1
]}

Chen, Ming-Hui ^{[1
]}

Sheng, Zhi-Chao ^{[2
]}

Liu, Dao-Feng ^{[1
]}

Wu, Song-Song ^{[1
]}

Lai, Wei-Hua ^{[1
]}

机构：

[1] Nanchang Univ, State Key Lab Food Sci & Technol, Nanchang 330047, Peoples R China

[2] Lianyungang Entry Exit Inspect & Quarantine Bur, Lianyungang 222000, Peoples R China

来源：

RSC ADVANCES | 2015年 / 5卷 / 76期

关键词：

RAPID DETECTION; QUANTITATIVE DETECTION; SWINE URINE; BIOSENSOR; ASSAY; CLENBUTEROL; LABEL; BEEF;

D O I：

10.1039/c5ra13279g

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A colloidal gold immunochromatographic signal-amplifying test strip with a sandwich format was successfully developed to detect Escherichia coli O157:H7. The detection signal was improved by addition of enhancers (HAuCl4 and NH2OH center dot HCl) to the nitrocellulose membrane of a conventional immunochromatographic strip. The detection sensitivities of the test strip before and after signal amplification were 4 x 10(4) and 5 x 10(3) CFU mL(-1), respectively, as determined by a colloidal gold strip reader. An 8-fold increase in sensitivity after signal amplification was accomplished within 20 min. No cross-reactivity with 27 non-target strains was observed, and the time for detecting E. coli O157:H7 in spiked milk samples was shortened by 1.5 h. The colloidal gold immunochromatographic signal-amplifying test strip thus provides a novel method for determining E. coli O157:H7 and is rapid, sensitive, convenient, and suitable for on-site detection.

引用

页码：62300 / 62305

页数：6

共 18 条

[1] Rapid Detection of Shiga Toxin-Producing Escherichia coli in Ground Beef by an Immunochromatography Kit in Combination with Short-Term Enrichment and Treatment for Shiga Toxin Release [J].

Arakawa, Yumi ;

Sawada, Takuo ;

Takatori, Kosuke ;

Lee, Ken-Ichi ;

Hara-Kudo, Yukiko .

BIOCONTROL SCIENCE, 2011, 16 (04) :159-164

[2] Enzyme-linked Immunosorbent assay of Escherichia coli O157:H7 in surface enhanced Poly(Methyl methacrylate) microchannels [J].

Bai, Yunling ;

Huang, Wei-Cho ;

Yang, Shang-Tian .

BIOTECHNOLOGY AND BIOENGINEERING, 2007, 98 (02) :328-339

[3] A sensitive chromatographic strip test for the rapid detection of enrofloxacin in chicken muscle [J].

Chen, Xuelan ;

Xu, Hengyi ;

Lai, Weihua ;

Chen, Yuan ;

Yang, Xiaohui ;

Xiong, Yonghua .

FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT, 2012, 29 (03) :383-391

[4] In-situ immuno-gold nanoparticle network ELISA biosensors for pathogen detection [J].

Cho, Il-Hoon ;

Irudayaraj, Joseph .

INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 2013, 164 (01) :70-75

[5] Label free polyaniline based impedimetric biosensor for detection of E. coli O157:H7 Bacteria [J].

Chowdhury, Ankan Dutta ;

De, Amitabha ;

Chaudhuri, Chirosree Roy ;

Bandyopadhyay, Krishnan ;

Sen, Pintu .

SENSORS AND ACTUATORS B-CHEMICAL, 2012, 171 :916-923

[6] Escherichia coli O157:H7: Animal Reservoir and Sources of Human Infection [J].

Ferens, Witold A. ;

Hovde, Carolyn J. .

FOODBORNE PATHOGENS AND DISEASE, 2011, 8 (04) :465-487

[7] Development of an immunochromatographic assay for rapid and quantitative detection of clenbuterol in swine urine [J].

Li, Chaohui ;

Luo, Wei ;

Xu, Hengyi ;

Zhang, Qin ;

Xu, Hong ;

Aguilar, Zoraida P. ;

Lai, Weihua ;

Wei, Hua ;

Xiong, Yonghua .

FOOD CONTROL, 2013, 34 (02) :725-732

[8] Gold immunochromatographic strips for enhanced detection of Avian influenza and Newcastle disease viruses [J].

Li, Jinfeng ;

Zou, Mingqiang ;

Chen, Yan ;

Xue, Qiang ;

Zhang, Fan ;

Li, Beibei ;

Wang, Yanfei ;

Qi, Xiaohua ;

Yang, Yi .

ANALYTICA CHIMICA ACTA, 2013, 782 :54-58

[9]

Ma ZF, 2002, ANGEW CHEM INT EDIT, V41, P2176, DOI 10.1002/1521-3773(20020617)41:12<2176::AID-ANIE2176>3.0.CO

[10]

2-X

← 1 2 →