In vitro viability and preservation of pollen grain of kiwi (Actinidia chinensis var. deliciosa (A. Chev.) A. Chev)

被引：22

作者：

Borghezan, Marcelo ^{[1
]}

Clauman, Alan David ^{[1
]}

Steinmacher, Douglas Andre ^{[2
]}

Guerra, Miguel Pedro ^{[1
]}

Orth, Afonso Inacio ^{[1
]}

机构：

[1] Univ Fed Santa Catarina, Dept Fitotecnia, CCA, BR-88040900 Florianopolis, SC, Brazil

[2] Inst Biosomat, BR-13825000 Holambra, SP, Brazil

来源：

CROP BREEDING AND APPLIED BIOTECHNOLOGY | 2011年 / 11卷 / 04期

关键词：

Tomuri; Matua; germination; cryopreservation; CITRUS POLLEN; GERMINATION; KIWIFRUIT; CONSERVATION;

D O I：

10.1590/S1984-70332011000400007

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Kiwi is a dioecious plant species, requiring cross pollination for fruit production. The objective of this study was to evaluate the in vitro viability and shelf life of pollen grains of two kiwi varieties. Flowers of the cultivars Matua and Tomuri were collected and the pollen germinated in vitro, in culture medium containing agar (1 %), sucrose (0, 5, 10, 20 and 40 %) and boric acid (0 and 50 mg L-1 H3BO3). Pollen grains were stored in a BOD incubator (25.0 degrees C), refrigerator (4.0 degrees C), freezer (-18.0 degrees C) and in liquid N-2 (-196.0 degrees C), and evaluated after 0, 40, 120, 240 and 365 days. The culture medium enriched with 12 % sucrose and 50 mg L-1 H3BO3 was the most suitable. Pollen grains can be stored for a short period in the refrigerator or freezer, and cryopreserved for at least one year.

引用

页码：338 / 344

页数：7

共 26 条

[1] Fruit production in kiwifruit (Actinidia deliciosa) using preserved pollen
Abreu, I
Oliveira, M
[J]. AUSTRALIAN JOURNAL OF AGRICULTURAL RESEARCH, 2004, 55 (05): : 565 - 569
[2] Bettiol Neto José Emilio, 2009, Bragantia, V68, P825, DOI 10.1590/S0006-87052009000400002
[3] Chagas Edvan Alves, 2010, Cienc. Rural, V40, P231, DOI 10.1590/S0103-84782010000200001
[4] IN VITRO GERMINATION OF POLLEN GRAINS IN Passiflora suberosa L. FOR ITS USE IN INTERSPECIFIC HYBRIDIZATION
Cruz, Thyane Viana
Souza, Margarete Magalhaes
Amaral Roza, Francis Valdo
Carvalho Viana, Americo Jose
De Oliveira Belo, Gabriela
Dos Santos Fonseca, Josefa Wilma
[J]. REVISTA BRASILEIRA DE FRUTICULTURA, 2008, 30 (04) : 875 - 879
[5] Dantas Adriana Cibele de Mesquita, 2005, Rev. Bras. Frutic., V27, P356, DOI 10.1590/S0100-29452005000300005
[6] Domingues E. T., 1999, Scientia Agricola, V56, P265, DOI 10.1590/S0103-90161999000200002
[7] Einhardt Patrícia Milech, 2006, Rev. Bras. Frutic., V28, P5, DOI 10.1590/S0100-29452006000100004
[8] Polyamine biosynthesis and control of the development of functional pollen in kiwifruit
Falasca, G.
Franceschetti, M.
Bagni, N.
Altamura, M. M.
Biasi, R.
[J]. PLANT PHYSIOLOGY AND BIOCHEMISTRY, 2010, 48 (07) : 565 - 573
[9] *FEPAGRO, 2011, KIW ALT AGR SERR GAU
[10] The need for characterisation and evaluation of germplasm: kiwifruit as an example
Ferguson, A. Ross
[J]. EUPHYTICA, 2007, 154 (03) : 371 - 382

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