The Drosophila nucleosome remodeling factor NURF is required for Ecdysteroid signaling and metamorphosis

被引：119
作者：
Badenhorst, P
Xiao, H
Cherbas, L
Kwon, SY
Voas, M
Rebay, I
Cherbas, P
Wu, C [1 ]
机构：
[1] NCI, Lab Mol Cell Biol, NIH, Bethesda, MD 20814 USA
[2] Univ Birmingham, Dept Anat, Inst Biomed Res, Birmingham B15 2TT, W Midlands, England
[3] Indiana Univ, Dept Biol, Bloomington, IN 47405 USA
[4] Indiana Univ, Ctr Genom & Bioinformat, Bloomington, IN 47405 USA
[5] MIT, Whitehead Inst, Cambridge, MA 02142 USA
来源：
GENES & DEVELOPMENT | 2005年 / 19卷 / 21期
基金：
英国惠康基金;
关键词：
NURF; chromatin remodeling; chromatin; Drosophila; ecdysteroid; ISWI;
D O I：
10.1101/gad.1342605
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
Drosophila NURF is an ISWI-containing ATP-dependent chromatin remodeling complex that regulates transcription by catalyzing nucleosome sliding. To determine in vivo gene targets of NURF, we performed whole genome expression analysis on mutants lacking the NURF-specific subunit NURF301. Strikingly, a large set of ecdysone-responsive targets is included among several hundred NURF-regulated genes. Null Nurf301 mutants do not undergo larval to pupal metamorphosis, and also enhance dominant-negative mutations in ecdysone receptor. Moreover, purified NURF binds EcR in an ecdysone-dependent manner, suggesting it is a direct effector of nuclear receptor activity. The conservation of NURF in mammals has broad implications for steroid signaling.
引用
收藏
页码：2540 / 2545
页数：6
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