Downregulation of IL-13 gene transcription by T-bet in human T cells

被引：6

作者：

Suzuki, Kazuya ^{[1
,3
]}

Kaminuma, Osamu ^{[1
]}

Hiroi, Takachika ^{[1
]}

Kitamura, Fujiko ^{[1
]}

Miyatake, Shoichiro ^{[2
]}

Takaiwa, Fumio ^{[3
]}

Tatsumi, Hideki ^{[4
]}

Nemoto, Soichi ^{[4
]}

Kitamura, Noriko ^{[5
]}

Mori, Akio ^{[5
]}

机构：

[1] Tokyo Metropolitan Inst Med Sci, Dept Allergy & Immunol, Tokyo 113, Japan

[2] Tokyo Metropolitan Inst Med Sci, Cytokine Project, Tokyo 113, Japan

[3] Natl Inst Agrobiol Sci, Transgen Crop Res & Dev Ctr, Tsukuba, Ibaraki, Japan

[4] Natl Hosp Org, Sagamihara Natl Hosp, Dept Obstet & Gynecol, Sagamihara, Kanagawa, Japan

[5] Natl Hosp Org, Sagamihara Natl Hosp, Clin Res Ctr Allergy & Rheumatol, Sagamihara, Kanagawa, Japan

来源：

INTERNATIONAL ARCHIVES OF ALLERGY AND IMMUNOLOGY | 2008年 / 146卷

关键词：

IL-13; T-bet; T cell; Th1/Th2;

D O I：

10.1159/000126058

中图分类号：

R392 [医学免疫学];

学科分类号：

100102 ;

摘要：

Background: Downregulation of a Th2 cytokine, IL-4, by a Th1-specific transcription factor, T-bet, has been demonstrated. However, the regulatory role of T-bet in another Th2 cytokine, IL-13, is not fully delineated. Methods: IL-13 mRNA expression in Jurkat cells was examined by quantitative RTPCR, while the transcriptional activity of 5'-flanking region in the IL-13 gene encompassing -1077 to +49 was investigated by fluorescence-based promoter reporter assay. The effect of T-bet was investigated by transfection of the cells with the T-bet expression vector. Results: Stimulation with phorbol ester plus Ca2+ ionophore clearly induced IL-13 gene transcription in Jurkat cells. Ectopically expressed T-bet significantly suppressed the inducible mRNA expression and promoter activity of IL-13. Conclusion: IL-13 expression was downregulated by T-bet at the level of gene transcription, independently of the modulation of Th1/Th2 balance. T-bet is the potential key factor in the development of Th1/Th2related diseases. Copyright (C) 2008 S. Karger AG, Basel.

引用

页码：33 / 35

页数：3

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