Downregulation of IL-13 gene transcription by T-bet in human T cells

被引：6
|
作者：
Suzuki, Kazuya [1 ,3 ]
Kaminuma, Osamu [1 ]
Hiroi, Takachika [1 ]
Kitamura, Fujiko [1 ]
Miyatake, Shoichiro [2 ]
Takaiwa, Fumio [3 ]
Tatsumi, Hideki [4 ]
Nemoto, Soichi [4 ]
Kitamura, Noriko [5 ]
Mori, Akio [5 ]
机构：
[1] Tokyo Metropolitan Inst Med Sci, Dept Allergy & Immunol, Tokyo 113, Japan
[2] Tokyo Metropolitan Inst Med Sci, Cytokine Project, Tokyo 113, Japan
[3] Natl Inst Agrobiol Sci, Transgen Crop Res & Dev Ctr, Tsukuba, Ibaraki, Japan
[4] Natl Hosp Org, Sagamihara Natl Hosp, Dept Obstet & Gynecol, Sagamihara, Kanagawa, Japan
[5] Natl Hosp Org, Sagamihara Natl Hosp, Clin Res Ctr Allergy & Rheumatol, Sagamihara, Kanagawa, Japan
来源：
INTERNATIONAL ARCHIVES OF ALLERGY AND IMMUNOLOGY | 2008年 / 146卷
关键词：
IL-13; T-bet; T cell; Th1/Th2;
D O I：
10.1159/000126058
中图分类号：
R392 [医学免疫学];
学科分类号：
100102 ;
摘要：
Background: Downregulation of a Th2 cytokine, IL-4, by a Th1-specific transcription factor, T-bet, has been demonstrated. However, the regulatory role of T-bet in another Th2 cytokine, IL-13, is not fully delineated. Methods: IL-13 mRNA expression in Jurkat cells was examined by quantitative RTPCR, while the transcriptional activity of 5'-flanking region in the IL-13 gene encompassing -1077 to +49 was investigated by fluorescence-based promoter reporter assay. The effect of T-bet was investigated by transfection of the cells with the T-bet expression vector. Results: Stimulation with phorbol ester plus Ca2+ ionophore clearly induced IL-13 gene transcription in Jurkat cells. Ectopically expressed T-bet significantly suppressed the inducible mRNA expression and promoter activity of IL-13. Conclusion: IL-13 expression was downregulated by T-bet at the level of gene transcription, independently of the modulation of Th1/Th2 balance. T-bet is the potential key factor in the development of Th1/Th2related diseases. Copyright (C) 2008 S. Karger AG, Basel.
引用
收藏
页码：33 / 35
页数：3
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