Background: Marked advances in artificial intelligence (AI)-based technologies throughout industries, including healthcare, necessitate a broader understanding their use. Particularly, intent to use AI-based healthcare technologies and its predictors among nursing students, who are prospective healthcare professionals, is required to promote the utilization of AI.Objective: This study conducted a path analysis to predict nursing students' intent to use AI-based healthcare technologies based on the unified theory of acceptance and use of technology.Design: A cross-sectional survey was performed.Participants: The participants were 210 nursing students from two nursing schools in Korea. Methods: This study established hypothetical paths for the influence of performance expectancy, effort expectancy, social influence, facilitating conditions, self-efficacy, and anxiety on intent to use AI-based technologies. Mediation of positive and negative attitudes and facilitating conditions' direct effects on intent to use were examined.Results: Positive attitude toward AI (13 = 0.485, p = .009) and facilitating conditions (13 = 0.117, p = .045) predicted intent to use, whereas the path from negative attitude to intent to use was not significant. Performance expectancy, self-efficacy, and effort expectancy predicted positive attitude. Performance expectancy and selfefficacy had a negative effect on the path to negative attitude, whereas anxiety had a positive effect. Facilitating conditions did not significantly predict positive or negative attitude and only directly predicted intent to use. Social influence did not have a significant effect on intent to use.Conclusions: Intervention programs and other measures should be developed to provide education and information to boost performance expectancy, effort expectancy, facilitating conditions, and self-efficacy regarding the use of AI to lower anxiety and foster positive attitude toward AI-based health technologies.