Optimizing rapid solvent exchange preparation of multilamellar vesicles

被引：11
|
作者：
Rieder, A. A. [1 ,2 ]
Koller, D. [1 ,2 ]
Lohner, K. [1 ,2 ]
Pabst, G. [1 ,2 ]
机构：
[1] Graz Univ, Inst Mol Biosci, Div Biophys, NAWI Graz, A-8010 Graz, Austria
[2] BioTechMed Graz, Graz, Austria
来源：
CHEMISTRY AND PHYSICS OF LIPIDS | 2015年 / 186卷
关键词：
Lipid vesicles; Diplamitoyl phosphatidylcholine; Micro-turbulences; Calorimetry; X-ray scattering; Photon correlation spectroscopy; LIPOSOMES; BEHAVIOR; PHASES;
D O I：
10.1016/j.chemphyslip.2014.12.001
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We have enhanced the rapid solvent exchange (RSE) apparatus by adding controls in temperature, evacuation speed and vortex velocity. Following published protocols yielded vesicles of diverse size and lamellarity as detected by differential scanning calorimetry, photon correlation spectroscopy and X-ray experiments. To optimize the net production of multilamellar vesicles (MLVs) we varied in addition to vortex and evacuation speed lipid and organic solvent concentration, as well as composition of the aqueous medium. Reducing vortexing frequencies and speed of degassing were most beneficial for the yield in MLVs. Additionally also high lipid concentrations and organic solvent/buffer ratios supported MLV formation. To explain our findings we hypothesize on the role of microscopic instabilities on the aqueous phase, which may act as molds for vesicle formation. (C) 2014 Elsevier Ireland Ltd. All rights reserved.
引用
收藏
页码：39 / 44
页数：6
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