Carboxylated chitosan enabled platinum nanozyme with improved stability and ascorbate oxidase-like activity for a fluorometric acid phosphatase sensor

被引:28
作者
He, Shao-Bin [1 ,2 ]
Yang, Liu [1 ]
Yang, Yu [1 ]
Noreldeen, Hamada A. A. [1 ]
Wu, Gang-Wei [1 ,3 ]
Peng, Hua-Ping [1 ]
Deng, Hao-Hua [1 ]
Chen, Wei [1 ]
机构
[1] Fujian Med Univ, Sch Pharm, Fujian Key Lab Drug Target Discovery & Struct & Fu, Fuzhou 350004, Peoples R China
[2] Fujian Med Univ, Dept Pharm, Lab Clin Pharm, Affiliated Hosp 2, Quanzhou 362000, Peoples R China
[3] Fujian Prov Hosp, Dept Pharm, Fuzhou 350001, Peoples R China
关键词
Carboxylation chitosan; Platinum; Nanozyme; Ascorbate oxidase-like activity; Fluorometric sensor; Acid phosphatase; NANOPARTICLES;
D O I
10.1016/j.carbpol.2022.120120
中图分类号
O69 [应用化学];
学科分类号
081704 ;
摘要
Chitosan modification has attracted considerable interest in the nanozyme field last decade. As a chitosan de-rivative, carboxylated chitosan (CC) has been less explored. Herein, PtNPs with an average size of approximately 3.3 nm and zeta potential of-44.8 +/- 0.3 mV (n = 3) have been prepared by using CC as the surface modification (CC-PtNPs). We have carried out an in-depth investigation of CC-PtNPs, including the characterization, colloidal stability, and ascorbate oxidase-like activity. Due to the contribution of carboxylated chitosan, CC-PtNPs present improved colloidal stability and ascorbate oxidase-like activity compared to chitosan-modified Pt nanozyme. Inspired by these results, a fluorometric acid phosphatase sensor was proposed based on the improved perfor-mance of CC-PtNPs. This sensor exhibits excellent sensitivity and selectivity towards acid phosphatase in the linear range of 0.25-18 U/L with a low limit of detection (1.31 x 10-3 U/L). The concentration of acid phos-phatase in human semen samples has been successfully measured.
引用
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页数:8
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