Studying DNA G-Quadruplex Aptamer by 19F NMR

被引：15

作者：

Ishizuka, Takumi ^{[1
]}

Yamashita, Atsushi ^{[2
]}

Asada, Yujiro ^{[2
]}

Xu, Yan ^{[1
]}

机构：

[1] Univ Miyazaki, Fac Med, Dept Med Sci, Div Chem, 5200 Kihara, Kiyotake, Miyazaki 8891692, Japan

[2] Univ Miyazaki, Div Pathophysiol, Dept Pathol, 5200 Kihara, Kiyotake, Miyazaki 8891692, Japan

来源：

ACS OMEGA | 2017年 / 2卷 / 12期

关键词：

RNA G-QUADRUPLEX; LIVING CELLS; SPECTROSCOPY; BINDING; THROMBIN; PROBES; ANTICOAGULANT; NUCLEOSIDE; STABILITY; MOLECULE;

D O I：

10.1021/acsomega.7b01405

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

In this study, we demonstrated that F-19 NMR can be used to study the thrombin-binding aptamer (TBA) DNA G-quadruplex, widely used as a model structure for studying G-quadruplex aptamers. We systematically examined the structural feature of the TBA G-quadruplex aptamer with fluorine-19 (F-19) labels at all of the thymidine positions. We successfully observed the structural change between the G-quadruplex and the unstructured single strand by F-19 NMR spectroscopy. The thermodynamic parameters of these DNA G-quadruplex aptamers were also determined from the F-19 NMR signals. We further showed that the F-19 NMR method can be used to observe the complex formed by TBA G-quadruplex and thrombin. Our results suggest that F-19 NMR spectroscopy is a useful approach to study the aptamer G-quadruplex structure.

引用

页码：8843 / 8848

页数：6

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