The melatonin action on stromal stem cells within pericryptal area in colon cancer model under constant light

被引：33

作者：

Kannen, Vinicius ^{[1
]}

Marini, Tassiana ^{[1
]}

Zanette, Dalila L. ^{[3
,4
]}

Frajacomo, Fernando T. ^{[1
]}

Silva, Gyl E. B. ^{[1
]}

Silva, Wilson A., Jr. ^{[2
,3
,4
]}

Garcia, Sergio B. ^{[1
]}

机构：

[1] Univ Sao Paulo, Med Sch Ribeirao Preto, Dept Pathol, BR-14049900 Ribeirao Preto, SP, Brazil

[2] Univ Sao Paulo, Med Sch Ribeirao Preto, Dept Genet, BR-14049900 Ribeirao Preto, SP, Brazil

[3] Ctr Cell Therapy, Natl Inst Sci & Technol Stem Cell & Cell Therapy, Ribeirao Preto, SP, Brazil

[4] Reg Blood Ctr, Ribeirao Preto, SP, Brazil

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2011年 / 405卷 / 04期

基金：

巴西圣保罗研究基金会;

关键词：

Colon cancer; Melatonin; Constant light; Cancer stem cells; Aberrant crypt foci; RATS; CARCINOGENESIS; APOPTOSIS; TARGET; 1,2-DIMETHYLHYDRAZINE; TUMORS; ANTAGONIZES; INCREASES; PATHWAY; ACID;

D O I：

10.1016/j.bbrc.2011.01.074

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Constant light (LL) is associated with high incidence of colon cancer. MLT supplementation was related to the significant control of preneoplastic patterns. We sought to analyze preneoplastic patterns in colon tissue from animals exposed to LL environment (14 days; 300 lx), MLT-supplementation (10 mg/kg/day) and DMH-treatment (1,2 dimethylhydrazine; 125 mg/kg). Rodents were sacrificed and MLT serum levels were measured by radioimmunoassay. Our results indicated that LL induced ACF development (p < 0.001) with a great potential to increase the number of CD133(+) and CD68(+) cells (p < 0.05 and p < 0.001). LL also increased the proliferative process (PCNA-Li; p < 0.001) as well as decreased caspase-3 protein (p < 0.001), related to higher COX-2 protein expression (p < 0.001) within pericryptal colonic stroma (PCCS). However, MLT-supplementation controlled the development of dysplastic ACF (p < 0.001) diminishing preneoplastic patterns into PCCS as CD133 and CD68 (p < 0.05 and p < 0.001). These events were relative to decreased PCNA-Li index and higher expression of caspase-3 protein. Thus, MLT showed a great potential to control the preneoplastic patterns induced by LL. (C) 2011 Elsevier Inc. All rights reserved.

引用

页码：593 / 598

页数：6