Microtubule nanospool formation by active self-assembly is not initiated by thermal activation

被引：27

作者：

Luria, Isaac ^{[2
]}

Crenshaw, Jasmine ^{[2
]}

Downs, Matthew ^{[1
,2
]}

Agarwal, Ashutosh ^{[1
,2
]}

Seshadri, Shruti Banavara ^{[2
]}

Gonzales, John ^{[1
]}

Idan, Ofer ^{[1
]}

Kamcev, Jovan ^{[1
]}

Katira, Parag ^{[2
]}

Pandey, Shivendra ^{[2
]}

Nitta, Takahiro ^{[3
]}

Phillpot, Simon R. ^{[2
]}

Hess, Henry ^{[1
]}

机构：

[1] Columbia Univ, Dept Biomed Engn, New York, NY 10027 USA

[2] Univ Florida, Dept Mat Sci & Engn, Gainesville, FL 32611 USA

[3] Gifu Univ, Dept Math & Design Engn, Gifu 5011193, Japan

来源：

SOFT MATTER | 2011年 / 7卷 / 07期

基金：

美国国家科学基金会;

关键词：

ASSISTED LIGHT INACTIVATION; MOLECULAR SHUTTLES; PERSISTENCE LENGTH; KINESIN; TRANSPORT; MOTORS; FLUCTUATIONS; RIGIDITY; FORCE; CARGO;

D O I：

10.1039/c0sm00802h

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Biotinylated microtubules partially coated with streptavidin and gliding on a surface coated with kinesin motors can cross-link with each other and assemble into nanospools with a diameter of a few micrometres. The size distribution of these nanospools is determined, and it is shown with simulations of microtubule gliding that these spools are too small to be formed by thermally activated turns in the gliding direction (a Brownian ratchet mechanism). Instead, spool formation is primarily the result of two processes: pinning of gliding microtubules to inactive motors and simultaneous cross-linking of multiple microtubules.

引用

页码：3108 / 3115

页数：8

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