Microtubule nanospool formation by active self-assembly is not initiated by thermal activation

被引：27

作者：

Luria, Isaac ^{[2
]}

Crenshaw, Jasmine ^{[2
]}

Downs, Matthew ^{[1
,2
]}

Agarwal, Ashutosh ^{[1
,2
]}

Seshadri, Shruti Banavara ^{[2
]}

Gonzales, John ^{[1
]}

Idan, Ofer ^{[1
]}

Kamcev, Jovan ^{[1
]}

Katira, Parag ^{[2
]}

Pandey, Shivendra ^{[2
]}

Nitta, Takahiro ^{[3
]}

Phillpot, Simon R. ^{[2
]}

Hess, Henry ^{[1
]}

机构：

[1] Columbia Univ, Dept Biomed Engn, New York, NY 10027 USA

[2] Univ Florida, Dept Mat Sci & Engn, Gainesville, FL 32611 USA

[3] Gifu Univ, Dept Math & Design Engn, Gifu 5011193, Japan

来源：

SOFT MATTER | 2011年 / 7卷 / 07期

基金：

美国国家科学基金会;

关键词：

ASSISTED LIGHT INACTIVATION; MOLECULAR SHUTTLES; PERSISTENCE LENGTH; KINESIN; TRANSPORT; MOTORS; FLUCTUATIONS; RIGIDITY; FORCE; CARGO;

D O I：

10.1039/c0sm00802h

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Biotinylated microtubules partially coated with streptavidin and gliding on a surface coated with kinesin motors can cross-link with each other and assemble into nanospools with a diameter of a few micrometres. The size distribution of these nanospools is determined, and it is shown with simulations of microtubule gliding that these spools are too small to be formed by thermally activated turns in the gliding direction (a Brownian ratchet mechanism). Instead, spool formation is primarily the result of two processes: pinning of gliding microtubules to inactive motors and simultaneous cross-linking of multiple microtubules.

引用

页码：3108 / 3115

页数：8

共 26 条

[1] Mechanisms Underlying the Active Self-Assembly of Microtubule Rings and Spools
VanDelinder, Virginia
Brener, Stephanie
Bachand, George D.
BIOMACROMOLECULES, 2016, 17 (03) : 1048 - 1056
[2] Cytoskeletal motor-driven active self-assembly in in vitro systems
Lam, A. T.
VanDelinder, V.
Kabir, A. M. R.
Hess, H.
Bachand, G. D.
Kakugo, A.
SOFT MATTER, 2016, 12 (04) : 988 - 997
[3] Active interface bulging in Bacillus subtilis swarms promotes self-assembly and biofilm formation
Liu, Siyu
Li, Ye
Xu, Haoran
Kearns, Daniel B.
Wu, Yilin
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2024, 121 (31)
[4] Formation of ring-shaped microtubule assemblies through active self-organization on dynein
Ito, Masaki
Kabir, Arif Md. Rashedul
Inoue, Daisuke
Torisawa, Takayuld
Toyoshima, Yoko
Sada, Kazuld
Kakugo, Akira
POLYMER JOURNAL, 2014, 46 (04) : 220 - 225
[5] Controlling self-assembly of microtubule spools via kinesin motor density
Lam, A. T.
Curschellas, C.
Krovvidi, D.
Hess, H.
SOFT MATTER, 2014, 10 (43) : 8731 - 8736
[6] A Cellular Automaton Approach to the Simulation of Active Self-Assembly of Kinesin-Powered Molecular Shuttles
Crenshaw, Jasmine D.
Liang, Tao
Hess, Henry
Phillpot, Simon R.
JOURNAL OF COMPUTATIONAL AND THEORETICAL NANOSCIENCE, 2011, 8 (10) : 1999 - 2005
[7] Magnetic Control of Protein Spatial Patterning to Direct Microtubule Self-Assembly
Hoffmann, Celine
Mazari, Elsa
Gosse, Charlie
Bonnennay, Louise
Hostachy, Sarah
Gautier, Jeremie
Gueroui, Zoher
ACS NANO, 2013, 7 (11) : 9647 - 9654
[8] Directed self-assembly of 1D microtubule nano-arrays
Bachand, M.
Bouxsein, N. F.
Cheng, S.
von Hoyningen-Huene, S. J.
Stevens, M. J.
Bachand, G. D.
RSC ADVANCES, 2014, 4 (97): : 54641 - 54649
[9] Nanoscale Transport Enables Active Self-Assembly of Millimeter-Scale Wires
Idan, Ofer
Lam, Amy
Kamcev, Jovan
Gonzales, John
Agarwal, Ashutosh
Hess, Henry
NANO LETTERS, 2012, 12 (01) : 240 - 245
[10] Self-Assembly of Catalytically Active Colloidal Molecules: Tailoring Activity Through Surface Chemistry
Soto, Rodrigo
Golestanian, Ramin
PHYSICAL REVIEW LETTERS, 2014, 112 (06)

← 1 2 3 →