Effect of Electro-acupuncture on Vasomotor Symptoms in Rats with Acute Cerebral Infarction Based on Phosphatidylinositol System

被引：11

作者：

Li Jing ^{[1
]}

He Ying ^{[1
]}

Du Yuan-hao ^{[2
]}

Zhang Min ^{[1
]}

Rainer, Georgi ^{[3
]}

Kolberg, Bernhard ^{[4
]}

Sun Dong-wei ^{[5
]}

Ma Kun ^{[6
]}

Li Yong-feng ^{[7
]}

Zhang Xue-zhu ^{[1
]}

机构：

[1] Tianjin Univ Tradit Chinese Med, Natl Clin Res Ctr Chinese Med Acupuncture & Moxib, Key Lab Acupuncture Tianjin, Teaching Hosp 1, Tianjin 300381, Peoples R China

[2] Tianjin Univ Tradit Chinese Med, Dept Acupuncture & Moxibust, Teaching Hosp 1, Tianjin 300381, Peoples R China

[3] Max Planck Inst Med Res, Dept Biol Chem, D-69120 Heidelberg, Germany

[4] Heidelberg Univ, Mannheim Med Sch, Dept Internal Med, D-68167 Mannheim, Germany

[5] Shenzhen Baoan Dist Hosp Tradit Chinese Med, Dept Chinese Med Rehabil, Shenzhen 518000, Guangdong, Peoples R China

[6] Tianjin Univ Tradit Chinese Med, Dept Prevent Treatment Dis, Binhai New Area Hosp Tradit Chinese Med, Teaching Hosp 4, Tianjin 300193, Peoples R China

[7] Shaanxi Univ Chinese Med, Inst Acupuncture & Moxibust, Xian 712046, Peoples R China

来源：

CHINESE JOURNAL OF INTEGRATIVE MEDICINE | 2022年 / 28卷 / 02期

基金：

中国国家自然科学基金;

关键词：

cerebral infarction; phosphatidylinositol system; vasomotion; Shuigou (GV 26); electro-acupuncture; Chinese medicine; ISCHEMIC BOUNDARY AREA; BLOOD-FLOW; ANGIOGENESIS; ACTIVATION; MECHANISMS; CELLS;

D O I：

10.1007/s11655-021-3341-6

中图分类号：

R [医药、卫生];

学科分类号：

10 ;

摘要：

Objective To investigate the effect of electro-acupuncture (EA) on vasomotor symptoms in rats with acute cerebral infarction, by observing the changes in the expression of factors related to the phosphatidylinositol (PI) system. Methods Forty-two Wistar rats were randomly divided into 3 groups by a random number table: the control group (n=6), the model group (n=18) and the EA group (n=18). The EA group was given EA treatment at Shuigou (GV 26) instantly after modeling with middle cerebral artery occlusion (MCAO) method, while the model and control groups were not given any treatment. The degrees of neurological deficiency were evaluated using neurological severity scores (NSS) and the brain blood flow was evaluated by a laser scanning confocal microscope. Western blot analysis was conducted to detect the expression levels of G-protein subtype (Gq) and calmodulin (CaM). Competition for protein binding was conducted to detect the expression level of inositol triphosphate (IP3). Thin layer quantitative analysis was conducted to detect the expression level of diacylglycerol (DAG). The expression level of intracellular concentration of free calcium ion ([Ca2+](i)) was detected by flow cytometry. Results The NSS of the model group was significantly higher than the control group at 3 and 6 h after MCAO (P<0.01), while the EA group was significantly lower than the model group at 6 h (P<0.01). The cerebral blood flow in the model group was significantly lower than the control group at 1, 3 and 6 h after MCAO (P<0.01), while for the EA group it was remarkably higher than the model group at the same time points (P<0.01). The expressions of Gq, CaM, IP3, DAG and [Ca2+](i) in the model group were significantly higher than the control group (PP<0.01), and those in the EA group were significantly lower than the model group at the same time points (PP<0.01). Conclusion EA treatment at GV 26 can effectively decrease the over-expression of related factors of PI system in rats with acute cerebral infarction, improve cerebral autonomy movement, and alleviate cerebral vascular spasm.

引用

页码：145 / 152

页数：8

共 31 条

[1] Calmodulin Binding to Connexin 35: Specializations to Function as an Electrical Synapse [J].