Casein kinase 2 and microtubules control axon initial segment formation

The axon initial segment (AIS) is a unique axonal subdomain responsible for the generation of the neuronal action potential and the maintenance of the axon-dendritic functional polarity. Despite its importance, the mechanisms controlling AIS development and maintenance remain largely unknown. Here we show that the AIS microtubule cytoskeleton is composed of a pool of more stable, detergent resistant, microtubules. This AIS specific characteristic is conferred by the presence of CK2, an important regulator of microtubule stability, in the AIS during its development and maturation. We show that CK2 alpha and CK2 alpha' subunits concentrate at the AIS from its initial development, at the same time as pI kappa B alpha and ankyrinG. CK2 pharmacological inhibition or suppression of CK2 alpha expression with nucleofected interference RNAs modifies microtubule characteristics throughout the neuron, changes KIF5C distribution, and impairs its own concentration at the AIS, as well as that of ankyrinG, ankyrinG-GFP, pI kappa B alpha and voltage gated sodium channels. Moreover, CK2 alpha concentration at the AIS depends on I kappa B alpha phosphorylation by IKK and ankyrinG. In conclusion, our results demonstrate a mutual dependence of CK2, ankyrinG and pI kappa B alpha for their concentration at the axon initial segment, which is related to the specific characteristics of microtubules at the AIS. (C) 2010 Elsevier Inc. All rights reserved.