Effect of γ-PGA on the formation of collagen fibrils in vitro

被引：14
|
作者：
Ding, Cuicui [1 ]
Zheng, Zhigong [1 ]
Liu, Xinzhong [1 ]
Li, Hengda [1 ]
Zhang, Min [2 ]
机构：
[1] Fujian Univ Technol, Coll Ecol Environm & Urban Construct, Fuzhou, Peoples R China
[2] Fujian Agr & Forestry Univ, Coll Mat Engn, Shangxiadian Rd 15, Fuzhou 350002, Peoples R China
来源：
CONNECTIVE TISSUE RESEARCH | 2016年 / 57卷 / 04期
基金：
中国国家自然科学基金;
关键词：
gamma-Poly(glutamic acid); collagen; fibril formation; hybrid hydrogels; self-assembly; MECHANICAL-PROPERTIES; PARAMETERS; SCAFFOLDS; ACID;
D O I：
10.3109/03008207.2016.1149170
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
The effect of gamma-poly(glutamic acid) (gamma-PGA) on the self-assembly of collagen was studied. Under physiological conditions, the kinetic curves for fibril formation showed that the turbidity of collagen/gamma-PGA blends at 313 nm was increased with the addition of gamma-PGA. Furthermore, it was shown using both field-emission scanning electron microscopy (FESEM) and atomic force microscopy (AFM) that fibrils with a larger diameter were obtained following the addition of gamma-PGA, probably due to the electrostatic and hydrogen bond interactions between collagen and gamma-PGA, which promoted the lateral association of collagen molecules. In addition, both the thermal stability and viscoelastic properties of the hybrid hydrogels, which were evaluated by differential scanning calorimetry and rheological measurements, respectively, were improved by the addition of gamma-PGA.
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页码：270 / 276
页数：7
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