Green synthesis of silver nanoparticles using transgenic Nicotiana tabacum callus culture expressing silicatein gene from marine sponge Latrunculia oparinae

被引:24
作者
Shkryl, Yuri N. [1 ]
Veremeichik, Galina N. [1 ]
Kamenev, Dmitriy G. [1 ]
Gorpenchenko, Tatiana Y. [1 ]
Yugay, Yulia A. [1 ]
Mashtalyar, Dmitriy V. [2 ]
Nepomnyaschiy, Aleksander V. [3 ]
Avramenko, Tatiana V. [1 ]
Karabtsov, Aleksandr A. [4 ]
Ivanov, Vladimir V. [4 ]
Bulgakov, Victor P. [1 ,5 ]
Gnedenkov, Sergey V. [2 ]
Kulchin, Yury N. [3 ]
Zhuravlev, Yury N. [1 ]
机构
[1] Russian Acad Sci, Dept Biotechnol, Fed Sci Ctr East Asia Terr Biodivers, Far East Branch, 159 Stoletija St, Vladivostok 690022, Russia
[2] Russian Acad Sci, Inst Chem, Far East Branch, Vladivostok, Russia
[3] Russian Acad Sci, Inst Automat & Control Proc, Far East Branch, Vladivostok, Russia
[4] Russian Acad Sci, Far East Geol Inst, Far East Branch, Vladivostok, Russia
[5] Far Eastern Fed Univ, Sch Nat Sci, Vladivostok, Russia
基金
俄罗斯科学基金会;
关键词
Silver nanoparticles; callus; silicatein; Nicotiana tabacum; green synthesis; antibacterial activity; METAL NANOPARTICLES; ANTIBACTERIAL ACTIVITY; ENZYMATIC-SYNTHESIS; BIOGENIC SYNTHESIS; PROTEIN; BIOSYNTHESIS; PLANT; EXTRACT; GOLD; SIZE;
D O I
10.1080/21691401.2017.1388248
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
In the present investigation, transgenic tobacco callus cultures and plants overexpressing the silicatein gene LoSilA1 from marine sponge Latrunculia oparinae were obtained and their bioreduction behaviour for the synthesis of silver nanoparticles (AgNPs) was studied. Synthesized nanoparticles were characterized using UV-visible spectroscopy, Fourier transformed infrared spectroscopy (FTIR), X-ray diffraction (XRD), scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDX), atomic flame electron microscopy (AFM) and nanoparticle tracking analysis (NTA). Our measurements showed that the reduction of silver nitrate produced spherical AgNPs with diameters in the range of 12-80 nm. The results of XRD analysis proved the crystal nature of the obtained AgNPs. FTIR analysis indicated that particles are reduced and stabilized in solution by the capping agent, which is likely to be proteins present in the callus extract. Interestingly, the reduction potential of LoSiLA1-transgenic callus line was increased three-fold compared with the empty vector-transformed calli. The synthesized AgNPs were found to exhibit strong antibacterial activity against Escherichia coli and Agrobacterium rhizogenes. The present study reports the first evidence for using genetic engineering for activation of the reduction potential of plant cells for synthesis of biocidal AgNPs.
引用
收藏
页码:1646 / 1658
页数:13
相关论文
共 100 条
[1]  
Abdin M Z., 2007, Natural Products-Essential resources for human survival, P45
[2]   Antibacterial efficacy of silver nanoparticles synthesized employing Terminalia arjuna bark extract [J].
Ahmed, Qadruddin ;
Gupta, Nidhi ;
Kumar, Ajeet ;
Nimesh, Surendra .
ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY, 2017, 45 (06) :1192-1200
[3]  
Akbari Vajihe, 2011, Avicenna Journal of Medical Biotechnology, V3, P167
[4]   Controlled formation of magnetite crystal by partial oxidation of ferrous hydroxide in the presence of recombinant magnetotactic bacterial protein Mms6 [J].
Amemiya, Yosuke ;
Arakaki, Atsushi ;
Staniland, Sarah S. ;
Tanaka, Tsuyoshi ;
Matsunaga, Tadashi .
BIOMATERIALS, 2007, 28 (35) :5381-5389
[5]   High-resolution atomic force microscopy studies of the Escherichia coli outer membrane:: Structural basis for permeability [J].
Amro, NA ;
Kotra, LP ;
Wadu-Mesthrige, K ;
Bulychev, A ;
Mobashery, S ;
Liu, GY .
LANGMUIR, 2000, 16 (06) :2789-2796
[6]  
[Anonymous], 2009, J Appl Biosci.
[7]  
[Anonymous], 2017, ARTIF CELLS NANOMED, DOI DOI 10.1080/21691401.2017.1304402
[8]   SilE is an intrinsically disordered periplasmic "molecular sponge" involved in bacterial silver resistance [J].
Asiani, Karishma R. ;
Williams, Huw ;
Bird, Louise ;
Jenner, Matthew ;
Searle, Mark S. ;
Hobman, Jon L. ;
Scott, David J. ;
Soultanas, Panos .
MOLECULAR MICROBIOLOGY, 2016, 101 (05) :731-742
[9]   Elucidating Protein Involvement in the Stabilization of the Biogenic Silver Nanoparticles [J].
Ballottin, Daniela ;
Fulaz, Stephanie ;
Souza, Michele L. ;
Corio, Paola ;
Rodrigues, Alexandre G. ;
Souza, Ana O. ;
Gaspari, Priscyla M. ;
Gomes, Alexandre F. ;
Gozzo, Fabio ;
Tasic, Ljubica .
NANOSCALE RESEARCH LETTERS, 2016, 11
[10]   Fungus-mediated biosynthesis of silica and titania particles [J].
Bansal, V ;
Rautaray, D ;
Bharde, A ;
Ahire, K ;
Sanyal, A ;
Ahmad, A ;
Sastry, M .
JOURNAL OF MATERIALS CHEMISTRY, 2005, 15 (26) :2583-2589