Modeling and immunohistochemical analysis of C6 glioma In Vivo

被引：35

作者：

Chekhonin, V. P. ^{[1
]}

Baklaushev, V. P. ^{[1
]}

Yusubalieva, G. M. ^{[1
]}

Pavlov, K. A. ^{[1
]}

Ukhova, O. V. ^{[1
]}

Gurina, O. I. ^{[1
]}

机构：

[1] Fed Agcy Hlth Care & Social Dev, VP Serbsky Natl Res Ctr Social & Forens Psychiat, Lab Immunochem, Moscow, Russia

来源：

BULLETIN OF EXPERIMENTAL BIOLOGY AND MEDICINE | 2007年 / 143卷 / 04期

关键词：

C6; glioma; immunohistochemistry of the brain; glia fibrillar acid protein; endothelial barrier antigen;

D O I：

10.1007/s10517-007-0167-y

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

A reproducible in vivo model of C6 glioma was developed in Wistar rats. Analysis of histological preparations showed similar morphology of rat C6 glioma and human glioblastoma. The formation of a glial border at the periphery of the glioma, consisting of GFAP-positive reactive astrocytes, was shown by the immunohistochemical method. The border appeared on day 8 after implantation, astrogliosis was observed until animal death (day 28). Reactive astrocytes with branched processes surrounded not only the primary glioma focus, but also all sites of tumor invasion in the nervous tissue. Expression of EBA (blood-brain barrier marker) was disturbed and synthesis of AMVB1 (endothelial antigen) increased in neoplastic endotheliocytes, which suggested pronounced functional restructuring of the blood-tumor barrier in comparison with the blood-brain barrier. The phenomenon of predominant expression of GFAP and AMVB1 in the tumor tissue can be used for the development of systems for targeted drug transport into the tumor by means of appropriate antibodies.

引用

页码：501 / 509

页数：9

共 23 条

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