The lepidopteran transposon vector, piggyBac, mediates germ-line transformation in the Mediterranean fruit fly

被引:208
作者
Handler, AM
McCombs, SD
Fraser, MJ
Saul, SH
机构
[1] USDA, ARS, Ctr Med Agr & Vet entomol, Gainesville, FL 32608 USA
[2] Univ Hawaii Manoa, Dept Entomol, Honolulu, HI 96822 USA
[3] Univ Notre Dame, Dept Biol Sci, Notre Dame, IN 46556 USA
关键词
D O I
10.1073/pnas.95.13.7520
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
The piggyBac (IFP2) short inverted terminal repeat transposable element from the cabbage looper Trichoplusia ni was tested for gene transfer vector function as part of a bipartite vector-helper system in the Mediterranean fruit fly Ceratitis capitata, A piggyBac vector marked with the medfly white gene was tested with a normally regulated piggyBac transposase helper at two different concentrations in a white eye host strain. Both experiments yielded transformants at an approximate frequency of 3-5%, with a total of six lines isolated having pigmented eyes with various levels of coloration, G(1) transformant siblings from each line shared at least one common integration, with several sublines having an additional second integration. For the first transformant line isolated, two integrations were determined to be stable for 15 generations. For five of the lines, a piggyBac-mediated transposition,vas verified by sequencing the insertion site junctions isolated by inverse PCR that identified a characteristic piggyBac TTAA target site duplication. The efficient and stable transformation of the medfly with a lepidopteran vector represents transposon function over a relatively large evolutionary distance and suggests that the piggyBac system will be functional in a broad range of insects.
引用
收藏
页码:7520 / 7525
页数:6
相关论文
共 40 条
[1]   BASIC LOCAL ALIGNMENT SEARCH TOOL [J].
ALTSCHUL, SF ;
GISH, W ;
MILLER, W ;
MYERS, EW ;
LIPMAN, DJ .
JOURNAL OF MOLECULAR BIOLOGY, 1990, 215 (03) :403-410
[2]   THE HOBO TRANSPOSABLE ELEMENT OF DROSOPHILA CAN BE CROSS-MOBILIZED IN HOUSEFLIES AND EXCISES LIKE THE AC ELEMENT OF MAIZE [J].
ATKINSON, PW ;
WARREN, WD ;
OBROCHTA, DA .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (20) :9693-9697
[3]   SEQUENCE COMPARISON OF CELLULAR AND VIRAL COPIES OF HOST-CELL DNA INSERTIONS FOUND IN AUTOGRAPHA-CALIFORNICA NUCLEAR POLYHEDROSIS-VIRUS [J].
BEAMES, B ;
SUMMERS, MD .
VIROLOGY, 1990, 174 (02) :354-363
[4]  
Cary L.C., 1989, Virology, V161, P8
[5]   Mariner transposition and transformation of the yellow fever mosquito, Aedes aegypti [J].
Coates, CJ ;
Jasinskiene, N ;
Miyashiro, L ;
James, AA .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (07) :3748-3751
[6]   PCR analysis of insertion site specificity, transcription, and structural uniformity of the Lepidopteran transposable element IFP2 in the TN-368 cell genome [J].
Elick, TA ;
Bauser, CA ;
Principe, NM ;
Fraser, MJ .
GENETICA, 1996, 97 (02) :127-139
[7]   Excision of the piggyBac transposable element in vitro is a precise event that is enhanced by the expression of its encoded transposase [J].
Elick, TA ;
Bauser, CA ;
Fraser, MJ .
GENETICA, 1996, 98 (01) :33-41
[8]   MOBILE MINOS ELEMENTS FROM DROSOPHILA-HYDEI ENCODE A 2-EXON TRANSPOSASE WITH SIMILARITY TO THE PAIRED DNA-BINDING DOMAIN [J].
FRANZ, G ;
LOUKERIS, TG ;
DIALEKTAKI, G ;
THOMPSON, CRL ;
SAVAKIS, C .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (11) :4746-4750
[9]   ACQUISITION OF HOST-CELL DNA-SEQUENCES BY BACULOVIRUSES - RELATIONSHIP BETWEEN HOST DNA INSERTIONS AND FP MUTANTS OF AUTOGRAPHA-CALIFORNICA AND GALLERIA-MELLONELLA NUCLEAR POLYHEDROSIS VIRUSES [J].
FRASER, MJ ;
SMITH, GE ;
SUMMERS, MD .
JOURNAL OF VIROLOGY, 1983, 47 (02) :287-300
[10]   ASSAY FOR MOVEMENT OF LEPIDOPTERAN TRANSPOSON IFP2 IN INSECT CELLS USING A BACULOVIRUS GENOME AS A TARGET DNA [J].
FRASER, MJ ;
CARY, L ;
BOONVISUDHI, K ;
WANG, HGH .
VIROLOGY, 1995, 211 (02) :397-407