European 2-A clonal complex of Mycobacterium bovis dominant in the Iberian Peninsula

被引：54

作者：

Rodriguez-Campos, Sabrina ^{[1
,2
]}

Schurch, Anita C. ^{[3
,4
]}

Dale, James ^{[5
]}

Lohan, Amanda J. ^{[6
]}

Cunha, Monica V. ^{[7
]}

Botelho, Ana ^{[7
]}

De Cruz, Krystel ^{[8
]}

Boschiroli, M. Laura ^{[8
]}

Boniotti, M. Beatrice ^{[9
]}

Pacciarini, Maria ^{[9
]}

Garcia-Pelayo, M. Carmen ^{[5
]}

Romero, Beatriz ^{[2
]}

de Juan, Lucia ^{[1
,2
]}

Dominguez, Lucas ^{[1
,2
]}

Gordon, Stephen V. ^{[10
,11
]}

van Soolingen, Dick ^{[3
,12
,13
]}

Loftus, Brendan ^{[6
]}

Berg, Stefan ^{[5
]}

Hewinson, R. Glyn ^{[5
]}

Aranaz, Alicia ^{[1
]}

Smith, Noel H. ^{[5
,14
]}

机构：

[1] Univ Complutense Madrid, Fac Vet, Dept Sanidad Anim, E-28040 Madrid, Spain

[2] Univ Complutense Madrid, Ctr Vigilancia Sanitaria Vet VISAVET, E-28040 Madrid, Spain

[3] Natl Inst Publ Hlth & Environm RIVM, Ctr Infect Dis Control, CIb LIS, TB Reference Lab, NL-3720 BA Bilthoven, Netherlands

[4] Radboud Univ Nijmegen, Med Ctr, NCMLS, Ctr Mol & Biomol Informat, NL-6500 HB Nijmegen, Netherlands

[5] Anim Hlth & Vet Labs Agcy, Addlestone KT15 3NB, Surrey, England

[6] Univ Coll Dublin, Conway Inst, Sch Med & Med Sci, Dublin 4, Ireland

[7] INRB, IP LNIV, Lab Nacl Invest Vet, P-1549011 Lisbon, Portugal

[8] Agence Natl Securite Sanit Alimentat Environm & T, Unite Zoonoses Bacteriennes, Lab Sante Anim Maisons Alfort, F-94706 Maisons Alfort, France

[9] Ist Zooprofilatt Sperimentale Lombardia & Emilia, Ctr Nazl Referenza TB Bovina, I-25124 Brescia, Italy

[10] Univ Coll Dublin, Coll Life Sci, Dublin 4, Ireland

[11] Univ Coll Dublin, UCD Conway Inst, Dublin 4, Ireland

[12] Radboud Univ Nijmegen, Med Ctr, Dept Clin Microbiol, NL-6500 HB Nijmegen, Netherlands

[13] Radboud Univ Nijmegen, Med Ctr, Dept Pulm Dis, NL-6500 HB Nijmegen, Netherlands

[14] Univ Sussex, Ctr Study Evolut, Brighton BN1 9QL, E Sussex, England

来源：

INFECTION GENETICS AND EVOLUTION | 2012年 / 12卷 / 04期

基金：

爱尔兰科学基金会;

关键词：

Mycobacterium bovis; Phylogeny; Europe; Whole genome sequencing; Clonal complex; DIRECT REPEAT LOCUS; STRAIN DIFFERENTIATION; EVOLUTIONARY SCENARIO; SPOLIGOTYPE DIVERSITY; MOLECULAR EVOLUTION; GENOMIC DELETIONS; TUBERCULOSIS; PHYLOGENY; GENES; IDENTIFICATION;

D O I：

10.1016/j.meegid.2011.09.004

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Mycobacterium bovis isolates from the Iberian Peninsula are dominated by strains with spoligotype patterns deleted for spacer 21. Whole-genome sequencing of three Spanish strains with spacer 21 missing in their spoligotype pattern revealed a series of SNPs and subsequent screening of a selection of these SNPs identified one in gene guaA that is specific to these strains. This group of strains from the Iberian Peninsula missing spoligotype spacer 21 represents a new clonal complex of M. bovis, defined by the SNP profile with a distinct spoligotype signature. We have named this clonal complex European 2 (Eu2) and found that it was present at low frequency in both France and Italy and absent from the British Isles. (C) 2011 Elsevier B.V. All rights reserved.

引用

页码：866 / 872

页数：7

共 38 条

[1] A historical and molecular phylogeny of BCG strains [J].

Behr, MA ;

Small, PM .

VACCINE, 1999, 17 (7-8) :915-922

[2] African 2, a Clonal Complex of Mycobacterium bovis Epidemiologically Important in East Africa [J].

Berg, Stefan ;

Garcia-Pelayo, M. Carmen ;

Mueller, Borna ;

Hailu, Elena ;

Asiimwe, Benon ;

Kremer, Kristin ;

Dale, James ;

Boniotti, M. Beatrice ;

Rodriguez, Sabrina ;

Hilty, Markus ;

Rigouts, Leen ;

Firdessa, Rebuma ;

Machado, Adelina ;

Mucavele, Custodia ;

Ngandolo, Bongo Nare Richard ;

Bruchfeld, Judith ;

Boschiroli, Laura ;

Mueller, Annelle ;

Sahraoui, Naima ;

Pacciarini, Maria ;

Cadmus, Simeon ;

Joloba, Moses ;

van Soolingen, Dick ;

Michel, Anita L. ;

Djonne, Berit ;

Aranaz, Alicia ;

Zinsstag, Jakob ;

van Helden, Paul ;

Portaels, Francoise ;

Kazwala, Rudovick ;

Kallenius, Gunilla ;

Hewinson, R. Glyn ;

Aseffa, Abraham ;

Gordon, Stephen V. ;

Smith, Noel H. .

JOURNAL OF BACTERIOLOGY, 2011, 193 (03) :670-678

[3] Molecular Typing of Mycobacterium bovis Strains Isolated in Italy from 2000 to 2006 and Evaluation of Variable-Number Tandem Repeats for Geographically Optimized Genotyping [J].

Boniotti, M. Beatrice ;

Goria, Maria ;

Loda, Daniela ;

Garrone, Annalisa ;

Benedetto, Alessandro ;

Mondo, Alessandra ;

Tisato, Ernesto ;

Zanoni, Mariagrazia ;

Zoppi, Simona ;

Dondo, Alessandro ;

Tagliabue, Silvia ;

Bonora, Stefano ;

Zanardi, Giorgio ;

Pacciarini, M. Lodovica .

JOURNAL OF CLINICAL MICROBIOLOGY, 2009, 47 (03) :636-644

[4] A new evolutionary scenario for the Mycobacterium tuberculosis complex [J].

Brosch, R ;

Gordon, SV ;

Marmiesse, M ;

Brodin, P ;

Buchrieser, C ;

Eiglmeier, K ;

Garnier, T ;

Gutierrez, C ;

Hewinson, G ;

Kremer, K ;

Parsons, LM ;

Pym, AS ;

Samper, S ;

van Soolingen, D ;

Cole, ST .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (06) :3684-3689

[5]

Craig DW, 2008, NAT METHODS, V5, P887, DOI [10.1038/nmeth.1251, 10.1038/NMETH.1251]

[6] Spoligotype diversity of Mycobacterium bovis and Mycobacterium caprae animal isolates [J].

Duarte, E. L. ;

Domingos, M. ;

Amado, A. ;

Botelho, A. .

VETERINARY MICROBIOLOGY, 2008, 130 (3-4) :415-421

[7] IS6110 transposition and evolutionary scenario of the direct repeat locus in a group of closely related Mycobacterium tuberculosis strains [J].

Fang, Z ;

Morrison, N ;

Watt, B ;

Doig, C ;

Forbes, KJ .

JOURNAL OF BACTERIOLOGY, 1998, 180 (08) :2102-2109

[8] Global phylogeny of Mycobacterium tuberculosis based on single nucleotide polymorphism (SNP) analysis:: Insights into tuberculosis evolution, phylogenetic accuracy of other DNA fingerprinting systems, and recommendations for a minimal standard SNP set [J].

Filliol, I ;

Motiwala, AS ;

Cavatore, M ;

Qi, WH ;

Hazbón, MH ;

del Valle, MB ;

Fyfe, J ;

García-García, L ;

Rastogi, N ;

Sola, C ;

Zozio, T ;

Guerrero, MI ;

León, CI ;

Crabtree, J ;

Angiuoli, S ;

Eisenach, KD ;

Durmaz, R ;

Joloba, ML ;

Rendón, A ;

Sifuentes-Osornio, J ;

de León, AP ;

Cave, MD ;

Fleischmann, R ;

Whittam, TS ;

Alland, D .

JOURNAL OF BACTERIOLOGY, 2006, 188 (02) :759-772

[9] Microarray analysis of Mycobacterium microti reveals deletion of genes encoding PE-PPE proteins and ESAT-6 family antigens [J].

Garcia-Pelayo, MC ;

Caimi, KC ;

Inwald, JK ;

Hinds, J ;

Bigi, F ;

Romano, MI ;

van Soolingen, D ;

Hewinson, RG ;

Cataldi, A ;

Gordon, SV .

TUBERCULOSIS, 2004, 84 (3-4) :159-166

[10] Identification of variable regions in the genomes of tubercle bacilli using bacterial artificial chromosome arrays [J].

Gordon, SV ;

Brosch, R ;

Billault, A ;

Garnier, T ;

Eiglmeier, K ;

Cole, ST .

MOLECULAR MICROBIOLOGY, 1999, 32 (03) :643-655

← 1 2 3 4 →