Overexpression of acetyl-CoA synthetase in Saccharomyces cerevisiae increases acetic acid tolerance

被引：46

作者：

Ding, Jun ^{[1
,2
,3
]}

Holzwarth, Garrett ^{[4
]}

Penner, Michael H. ^{[2
]}

Patton-Vogt, Jana ^{[5
]}

Bakalinsky, Alan T. ^{[1
,2
]}

机构：

[1] Oregon State Univ, Dept Biochem & Biophys, Corvallis, OR 97331 USA

[2] Oregon State Univ, Dept Food Sci & Technol, Corvallis, OR 97331 USA

[3] Res & Dev Medolac Labs, Lake Oswego, OR 97035 USA

[4] Oregon State Univ, Dept Microbiol, Corvallis, OR 97331 USA

[5] Duquesne Univ, Dept Biol Sci, Pittsburgh, PA 15282 USA

来源：

FEMS MICROBIOLOGY LETTERS | 2015年 / 362卷 / 03期

基金：

美国农业部; 美国国家卫生研究院;

关键词：

Saccharomyces cerevisiae; acetyl-CoA synthetase; Acs2; acetic acid; lignocellulose; COENZYME-A SYNTHETASE; PYRUVATE-DEHYDROGENASE BYPASS; PROGRAMMED CELL-DEATH; YEAST; ACETATE; GROWTH; GENE; GLUCOSE; INVOLVEMENT; INHIBITION;

D O I：

10.1093/femsle/fnu042

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Acetic acid-mediated inhibition of the fermentation of lignocellulose-derived sugars impedes development of plant biomass as a source of renewable ethanol. In order to overcome this inhibition, the capacity of Saccharomyces cerevisiae to synthesize acetyl-CoA from acetic acid was increased by overexpressing ACS2 encoding acetyl-coenzyme A synthetase. Overexpression of ACS2 resulted in higher resistance to acetic acid as measured by an increased growth rate and shorter lag phase relative to a wild-type control strain, suggesting that Acs2-mediated consumption of acetic acid during fermentation contributes to acetic acid detoxification.

引用

页数：7

共 39 条

[1] Yeast protein expression profile during acetic acid-induced apoptosis indicates causal involvement of the TOR pathway [J].

Almeida, Bruno ;

Ohlmeier, Steffen ;

Almeida, Agostinho J. ;

Madeo, Frank ;

Leao, Cecilia ;

Rodrigues, Fernando ;

Ludovico, Paula .

PROTEOMICS, 2009, 9 (03) :720-732

[2]

[Anonymous], 2002, Tech. Rep. NREL/TP-510-32438

[3] Signaling of Chloroquine-Induced Stress in the Yeast Saccharomyces cerevisiae Requires the Hog1 and Slt2 Mitogen-Activated Protein Kinase Pathways [J].

Baranwal, Shivani ;

Azad, Gajendra Kumar ;

Singh, Vikash ;

Tomar, Raghuvir S. .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2014, 58 (09) :5552-5566

[4]

BERG P, 1956, J BIOL CHEM, V222, P991

[5]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[6]

Chen Fujiang, 2010, Wei Sheng Wu Xue Bao, V50, P1172

[7] Establishing a platform cell factory through engineering of yeast acetyl-CoA metabolism [J].

Chen, Yun ;

Daviet, Laurent ;

Schalk, Michel ;

Siewers, Verena ;

Nielsen, Jens .

METABOLIC ENGINEERING, 2013, 15 :48-54

[8] Overproduction of acetyl-coenzyme A synthetase isoenzymes in respiring Saccharomyces cerevisiae cells does not reduce acetate production after exposure to glucose excess [J].

de Jong-Gubbels, P ;

van den Berg, MA ;

Luttick, MAH ;

Steensma, HY ;

van Dijken, JP ;

Pronk, JT .

FEMS MICROBIOLOGY LETTERS, 1998, 165 (01) :15-20

[9] Occurrence of naturally acetylated lignin units [J].

Del Rio, Jose C. ;

Marques, Gisela ;

Rencoret, Jorge ;

Martinez, Angel T. ;

Gutierrez, Ana .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2007, 55 (14) :5461-5468

[10] CLONING AND DISRUPTION OF A GENE REQUIRED FOR GROWTH ON ACETATE BUT NOT ON ETHANOL - THE ACETYL-COENZYME-A SYNTHETASE GENE OF SACCHAROMYCES-CEREVISIAE [J].

DEVIRGILIO, C ;

BURCKERT, N ;

BARTH, G ;

NEUHAUS, JM ;

BOLLER, T ;

WIEMKEN, A .

YEAST, 1992, 8 (12) :1043-1051

← 1 2 3 4 →