A multiplexed set of microsatellite markers for discriminating Acacia mangium, A-auriculiformis, and their hybrid

被引：16

作者：

Le, Son ^{[1
,2
,4
]}

Ratnam, Wickneswari

Harwood, Christopher E. ^{[1
,3
]}

Larcombe, Matthew J. ^{[1
]}

Griffin, Rod A. ^{[1
]}

Koutoulis, Anthony ^{[1
]}

Harbard, Jane L. ^{[1
]}

Cyer, Koh Sin ^{[2
]}

Yee, Liew Wai ^{[2
]}

Thinh Huy Ha ^{[4
]}

Vaillancourt, Rene E. ^{[1
]}

机构：

[1] Univ Tasmania, Sch Biol Sci, Private Bag 55, Hobart, Tas 7001, Australia

[2] Univ Kebangsaan Malaysia, Fac Sci & Technol, Bangi 43600, Selangor, Malaysia

[3] CSIRO Land & Water, Private Bag 12, Hobart, Tas 7001, Australia

[4] Vietnamese Acad Forest Sci, Hanoi, Vietnam

来源：

TREE GENETICS & GENOMES | 2016年 / 12卷 / 02期

关键词：

Population genetics; Breeding population; Hybrid discrimination; Microsatellite; CROSS-SPECIES AMPLIFICATION; DETECTING HYBRIDIZATION; SOFTWARE; POPULATIONS; LEGUMINOSAE; INDIVIDUALS;

D O I：

10.1007/s11295-016-0990-2

中图分类号：

S7 [林业];

学科分类号：

0829 ; 0907 ;

摘要：

In order to assist breeding and gene pool conservation in tropical Acacias, we aimed to develop a set of multipurpose SSR markers for use in both Acacia mangium and A. auriculiformis. A total of 51 SSR markers (developed in A. mangium and natural A. mangium x A. auriculiformis hybrid) were tested. A final set of 16 well-performing SSR markers were identified, six of which were species diagnostic. The markers were optimized for assay in four multiplex mixes and used to genotype range-wide samples of A. mangium, A. auriculiformis, and putative F-1 hybrids. Simulation analysis was used to investigate the power of the markers for identifying the pure species and their F-1, F-2, and backcross hybrids. The six species diagnostic markers were particularly powerful for detecting F-1 hybrids from pure species but could also discriminate the pure species from F-2 and backcross progenies in most cases (97 %). STRUCTURE analysis using all 16 markers was likewise able to distinguish these cross types and pure species sets. Both sets of markers had difficulties in distinguishing F-2 and backcross progenies. However, identifying F-1 from pure species is the current primary concern in countries where these species are planted. The SSR marker set also has direct application in DNA profiling (probability of identity=4.1 x 10(-13)), breeding system analysis, and population genetics.

引用

页数：10