The cytoskeleton of the myenteric neurons during murine embryonic life

被引:17
作者
Faussone-Pellegrini, MS
Matini, P
DeFelici, M
机构
[1] Univ Florence, Dept Human Anat & Histol, Sect Histol Enrico Allara, I-50139 Florence, Italy
[2] Univ Roma Tor Vergata, Dept Publ Hlth & Cell Biol, Sect Histol & Embriol, Rome, Italy
来源
ANATOMY AND EMBRYOLOGY | 1999年 / 199卷 / 05期
关键词
differentiation; electron microscopy; histochemistry; microtubules; neurofilaments;
D O I
10.1007/s004290050244
中图分类号
R602 [外科病理学、解剖学]; R32 [人体形态学];
学科分类号
100101 ;
摘要
The organization of the cytoskeleton has been studied during mouse differentiation in cells of the myenteric neuronal lineage. The entire gut was examined starting from day 12.5 of embryonic life (E12.5) until birth (PO). Immunocytochemistry was performed to evaluate the expression of five of the most represented neurofilaments proteins (the low, NF-L, medium, NF-M, and heavy, NF-H, molecular weight subunits, alpha-internexin and peripherin) and of two of the microtubule-associated proteins (MAP1 and MAP2a+2b). In parallel, the appearance in the differentiating myenteric neurons of filamentous and microtubular structures and their intracyto-plasmatic distribution were observed under the electron microscope. A differential immunohistochemical expression of the structural proteins was found. Immature cells expressed a-internexin, peripherin, NF-M and MAP1 by day E12.5; a-internexin expression was strong in these cells, but gradually decreased with age and was practically absent in adulthood. Conversely, the expression of the other three proteins increased with cell differentiation and was still present in adulthood. NF-L and NF-H expression appeared later, by day E16.5, and was weak for the entire pre- and postnatal life. MAP2a+2b was never expressed. Under the electron microscope, at day E12.5 the cytoskeleton was already organized in filamentous and microtubular structures. At this age neurofilaments were few and mainly located in the cell processes, and microtubules were numerous and mainly assembled in the neuritic growth cones, together with synaptic vesicles. With ageing, neurofilaments and microtubules were ubiquitous in the neuron. Data obtained demonstrate that cytoskeletal proteins gradually accumulate in the cells of the neuronal lineage in parallel with the organization of the cytoskeletal structures, which in turn mediate important neural events by the earliest stages of murine embryonic life, including growth of nerve processes and initiation of axonal transport.
引用
收藏
页码:459 / 469
页数:11
相关论文
共 29 条
[1]  
BISHOP AE, 1985, HISTOCHEMISTRY, V8, P293
[2]   NEUROFILAMENT AND GLIAL FIBRILLARY ACID PROTEIN-RELATED IMMUNOREACTIVITY IN RODENT ENTERIC NERVOUS-SYSTEM [J].
BJORKLUND, H ;
DAHL, D ;
SEIGER, A .
NEUROSCIENCE, 1984, 12 (01) :277-287
[3]   ASSOCIATION OF MICROTUBULE-ASSOCIATED PROTEIN-2 (MAP-2) WITH MICROTUBULES AND INTERMEDIATE FILAMENTS IN CULTURED BRAIN-CELLS [J].
BLOOM, GS ;
VALLEE, RB .
JOURNAL OF CELL BIOLOGY, 1983, 96 (06) :1523-1531
[4]   ASSEMBLY OF TYPE-IV NEURONAL INTERMEDIATE FILAMENTS IN NONNEURONAL CELLS IN THE ABSENCE OF PREEXISTING CYTOPLASMIC INTERMEDIATE FILAMENTS [J].
CHING, GY ;
LIEM, RKH .
JOURNAL OF CELL BIOLOGY, 1993, 122 (06) :1323-1335
[5]  
COCHARD P, 1984, J NEUROSCI, V4, P2080
[6]   THE DISTRIBUTION OF NOVEL INTERMEDIATE FILAMENT PROTEINS DEFINES SUBPOPULATIONS OF MYENTERIC NEURONS IN RAT INTESTINE [J].
EAKER, EY ;
SALLUSTIO, JE .
GASTROENTEROLOGY, 1994, 107 (03) :666-674
[7]   NEUROFILAMENT IMMUNOREACTIVITY IN MYENTERIC NEURONS DIFFERS FROM THAT FOUND IN THE CENTRAL-NERVOUS-SYSTEM [J].
EAKER, EY ;
SHAW, G ;
SNINSKY, CA .
GASTROENTEROLOGY, 1990, 99 (05) :1364-1371
[8]   MYENTERIC PLEXUS NEURONS HAVE DEVELOPMENTALLY ACQUIRED DIFFERENCES IN THE MEDIUM MOLECULAR-WEIGHT SUBUNIT OF NEUROFILAMENT PROTEIN [J].
EAKER, EY ;
SALLUSTIO, JE ;
HARRIS, JM ;
SHAW, G .
NEUROSCIENCE, 1993, 53 (02) :561-570
[9]  
Eaker EY, 1997, GASTROENTEROLOGY, V112, pA726
[10]   FORMATION OF 10-NANOMETER FILAMENTS FROM THE 150K-DALTON NEUROFILAMENT PROTEIN INVITRO [J].
GARDNER, EE ;
DAHL, D ;
BIGNAMI, A .
JOURNAL OF NEUROSCIENCE RESEARCH, 1984, 11 (02) :145-155