trans- Splicing, in which a spliced- leader ( SL) RNA is appended to the most 5' exon of independently transcribed pre-mRNAs, has been described in a wide range of eukaryotes, from protozoans to chordates. Here we describe trans- splicing in the freshwater planarian Schmidtea mediterranea, a free- living member of the phylum Platyhelminthes. Analysis of an expressed sequence tag ( EST) collection from this organism showed that over 300 transcripts shared one of two similar to 35- base sequences ( Smed SL- 1 and SL- 2) at their 5# ends. Examination of genomic sequences encoding representatives of these transcripts revealed that these shared sequences were transcribed elsewhere in the genome. RNA blot analysis, 5' and 3' rapid amplification of cDNA ends, as well as genomic sequence data showed that 42- nt SL sequences were derived from small RNAs of; 110 nt. Similar sequences were also found at the 5' ends of ESTs from the planarian Dugesia japonica. trans- Splicing has already been described in numerous representatives of the phylum Platyhelminthes ( trematodes, cestodes, and polyclads); its presence in two representatives of the triclads supports the hypothesis that this mode of RNA processing is ancestral within this group. The upcoming complete genome sequence of S. mediterranea, combined with this animal's experimental accessibility and susceptibility to RNAi, provide another model organism in which to study the function of the still- enigmatic trans- splicing.
机构:
Bar Ilan Univ, Mina & Everard Goodman Fac Life Sci, IL-52900 Ramat Gan, Israel
Bar Ilan Univ, Adv Mat & Nanotechnol Inst, IL-52900 Ramat Gan, IsraelBar Ilan Univ, Mina & Everard Goodman Fac Life Sci, IL-52900 Ramat Gan, Israel
Shaked, Hadassa
Wachtel, Chaim
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Bar Ilan Univ, Mina & Everard Goodman Fac Life Sci, IL-52900 Ramat Gan, Israel
Bar Ilan Univ, Adv Mat & Nanotechnol Inst, IL-52900 Ramat Gan, IsraelBar Ilan Univ, Mina & Everard Goodman Fac Life Sci, IL-52900 Ramat Gan, Israel
Wachtel, Chaim
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Tulinski, Pawel
Yahia, Nasreen Hag
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Bar Ilan Univ, Mina & Everard Goodman Fac Life Sci, IL-52900 Ramat Gan, Israel
Bar Ilan Univ, Adv Mat & Nanotechnol Inst, IL-52900 Ramat Gan, IsraelBar Ilan Univ, Mina & Everard Goodman Fac Life Sci, IL-52900 Ramat Gan, Israel
Yahia, Nasreen Hag
Barda, Omer
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Bar Ilan Univ, Mina & Everard Goodman Fac Life Sci, IL-52900 Ramat Gan, Israel
Bar Ilan Univ, Adv Mat & Nanotechnol Inst, IL-52900 Ramat Gan, IsraelBar Ilan Univ, Mina & Everard Goodman Fac Life Sci, IL-52900 Ramat Gan, Israel
Barda, Omer
Darzynkiewicz, Edward
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Univ Warsaw, Inst Expt Phys, Dept Biophys, PL-02089 Warsaw, PolandBar Ilan Univ, Mina & Everard Goodman Fac Life Sci, IL-52900 Ramat Gan, Israel
Darzynkiewicz, Edward
Nilsen, Timothy W.
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Case Western Reserve Univ, Sch Med, Ctr RNA Mol Biol, Cleveland, OH 44106 USABar Ilan Univ, Mina & Everard Goodman Fac Life Sci, IL-52900 Ramat Gan, Israel
Nilsen, Timothy W.
Michaeli, Shulamit
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Bar Ilan Univ, Mina & Everard Goodman Fac Life Sci, IL-52900 Ramat Gan, Israel
Bar Ilan Univ, Adv Mat & Nanotechnol Inst, IL-52900 Ramat Gan, IsraelBar Ilan Univ, Mina & Everard Goodman Fac Life Sci, IL-52900 Ramat Gan, Israel
机构:
Univ Calif Los Angeles, Sch Med, Dept Microbiol & Immunol, Los Angeles, CA 90095 USAUniv Calif Los Angeles, Sch Med, Dept Microbiol & Immunol, Los Angeles, CA 90095 USA
Sturm, NR
Campbell, DK
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Univ Calif Los Angeles, Sch Med, Dept Microbiol & Immunol, Los Angeles, CA 90095 USAUniv Calif Los Angeles, Sch Med, Dept Microbiol & Immunol, Los Angeles, CA 90095 USA
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Univ Paris 06, Observ Oceanol, Biol Dev UMR 7138, F-06230 Villefranche Sur Mer, France
CNRS, F-06230 Villefranche Sur Mer, FranceUniv Paris 06, Observ Oceanol, Biol Dev UMR 7138, F-06230 Villefranche Sur Mer, France
Derelle, Romain
Momose, Tsuyoshi
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Univ Paris 06, Observ Oceanol, Biol Dev UMR 7138, F-06230 Villefranche Sur Mer, France
CNRS, F-06230 Villefranche Sur Mer, FranceUniv Paris 06, Observ Oceanol, Biol Dev UMR 7138, F-06230 Villefranche Sur Mer, France
Momose, Tsuyoshi
Manuel, Michael
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Univ Paris 06, UMR 7138, F-75005 Paris, France
CNRS, F-75005 Paris, FranceUniv Paris 06, Observ Oceanol, Biol Dev UMR 7138, F-06230 Villefranche Sur Mer, France
Manuel, Michael
Da Silva, Corinne
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Ctr Natl Sequencage, Genoscope, F-91057 Evry, FranceUniv Paris 06, Observ Oceanol, Biol Dev UMR 7138, F-06230 Villefranche Sur Mer, France
Da Silva, Corinne
Wincker, Patrick
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Ctr Natl Sequencage, Genoscope, F-91057 Evry, FranceUniv Paris 06, Observ Oceanol, Biol Dev UMR 7138, F-06230 Villefranche Sur Mer, France
Wincker, Patrick
Houliston, Evelyn
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Univ Paris 06, Observ Oceanol, Biol Dev UMR 7138, F-06230 Villefranche Sur Mer, France
CNRS, F-06230 Villefranche Sur Mer, FranceUniv Paris 06, Observ Oceanol, Biol Dev UMR 7138, F-06230 Villefranche Sur Mer, France
机构:
Univ Tokyo, Inst Med Sci, Human Genome Ctr, Tokyo 1088639, JapanUniv Tokyo, Inst Med Sci, Human Genome Ctr, Tokyo 1088639, Japan
Yokomori, Rui
Kusakabe, Takehiro G.
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Konan Univ, Inst Integrat Neurobiol, Grad Sch Nat Sci, Kobe 6588501, Japan
Konan Univ, Fac Sci & Engn, Dept Biol, Kobe 6588501, JapanUniv Tokyo, Inst Med Sci, Human Genome Ctr, Tokyo 1088639, Japan
Kusakabe, Takehiro G.
Nakai, Kenta
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Univ Tokyo, Inst Med Sci, Human Genome Ctr, Tokyo 1088639, JapanUniv Tokyo, Inst Med Sci, Human Genome Ctr, Tokyo 1088639, Japan