Protective effect of chitosan oligosaccharides against FcεRI-mediated RBL-2H3 mast cell activation

被引：22
作者：
Vo, Thanh-Sang [2 ]
Kim, Jung-Ae [2 ]
Ngo, Dai-Hung [2 ]
Kong, Chang-Suk [1 ]
Kim, Se-Kwon [1 ,2 ]
机构：
[1] Pukyong Natl Univ, Marine Bioproc Res Ctr, Pusan 608737, South Korea
[2] Pukyong Natl Univ, Dept Chem, Pusan 608737, South Korea
来源：
PROCESS BIOCHEMISTRY | 2012年 / 47卷 / 02期
关键词：
Chitosan oligosaccharides; Histamine; Mast cells; Intracellular Ca2+; Fc epsilon RI; EXPRESSION; DEGRANULATION;
D O I：
10.1016/j.procbio.2011.10.036
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The activation of mast cells by immunoglobulin E-mediated stimuli is considered as a central event in allergic responses. In this regard, chitosan oligosaccharides (COS) of two different molecular weight ranges (1-3 kDa and 3-5 kDa) were investigated for their capabilities against the activation of RBL-2H3 mast cell sensitized with dinitrophenyl-specific immunoglobulin E antibody and stimulated by antigen dinitrophenyl-bovine serum albumin. It was found that COS significantly inhibited RBL-2H3 cell degranulation via attenuating the releases of histamine and beta-hexosaminidase. Moreover, the inhibitory activity of COS was accompanied by a reduction in intracellular Ca2+ elevation. Notably, the expression of immunoglobulin Fc epsilon receptor I (Fc epsilon RI) in RBL-2H3 cells was down-regulated by COS treatment in a dose-dependent manner. The suppressive effect of COS on RBL-2H3 cell activation suggested that COS may be potential candidates of novel inhibitors against allergic reactions. (C) 2011 Elsevier Ltd. All rights reserved.
引用
收藏
页码：327 / 330
页数：4
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