Protective effect of chitosan oligosaccharides against FcεRI-mediated RBL-2H3 mast cell activation

被引：22

作者：

Vo, Thanh-Sang ^{[2
]}

Kim, Jung-Ae ^{[2
]}

Ngo, Dai-Hung ^{[2
]}

Kong, Chang-Suk ^{[1
]}

Kim, Se-Kwon ^{[1
,2
]}

机构：

[1] Pukyong Natl Univ, Marine Bioproc Res Ctr, Pusan 608737, South Korea

[2] Pukyong Natl Univ, Dept Chem, Pusan 608737, South Korea

来源：

PROCESS BIOCHEMISTRY | 2012年 / 47卷 / 02期

关键词：

Chitosan oligosaccharides; Histamine; Mast cells; Intracellular Ca2+; Fc epsilon RI; EXPRESSION; DEGRANULATION;

D O I：

10.1016/j.procbio.2011.10.036

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The activation of mast cells by immunoglobulin E-mediated stimuli is considered as a central event in allergic responses. In this regard, chitosan oligosaccharides (COS) of two different molecular weight ranges (1-3 kDa and 3-5 kDa) were investigated for their capabilities against the activation of RBL-2H3 mast cell sensitized with dinitrophenyl-specific immunoglobulin E antibody and stimulated by antigen dinitrophenyl-bovine serum albumin. It was found that COS significantly inhibited RBL-2H3 cell degranulation via attenuating the releases of histamine and beta-hexosaminidase. Moreover, the inhibitory activity of COS was accompanied by a reduction in intracellular Ca2+ elevation. Notably, the expression of immunoglobulin Fc epsilon receptor I (Fc epsilon RI) in RBL-2H3 cells was down-regulated by COS treatment in a dose-dependent manner. The suppressive effect of COS on RBL-2H3 cell activation suggested that COS may be potential candidates of novel inhibitors against allergic reactions. (C) 2011 Elsevier Ltd. All rights reserved.

引用

页码：327 / 330

页数：4

共 24 条

[1] Production of Chitooligosaccharides and Their Potential Applications in Medicine
Aam, Berit B.
Heggset, Ellinor B.
Norberg, Anne Line
Sorlie, Morten
Varum, Kjell M.
Eijsink, Vincent G. H.
[J]. MARINE DRUGS, 2010, 8 (05) : 1482 - 1517
[2] Correlation between cytosolic calcium concentration and degranulation in RBL-2H3 cells in the presence of various concentrations of antigen-specific IgEs
Aketani, S
Teshima, R
Umezawa, Y
Sawada, J
[J]. IMMUNOLOGY LETTERS, 2001, 75 (03) : 185 - 189
[3] The mast cell and allergic diseases: role in pathogenesis and implications for therapy
Brown, J. M.
Wilson, T. M.
Metcalfe, D. D.
[J]. CLINICAL AND EXPERIMENTAL ALLERGY, 2008, 38 (01) : 4 - 18
[4] Influence of molecular weight on oral absorption of water soluble chitosans
Chae, SY
Jang, MK
Nah, JW
[J]. JOURNAL OF CONTROLLED RELEASE, 2005, 102 (02) : 383 - 394
[5] Lipid raft-associated catechin suppresses the FcεRI expression by inhibiting phosphorylation of the extracellular signal-regulated kinase1/2
Fujimura, Y
Tachibana, H
Yamada, K
[J]. FEBS LETTERS, 2004, 556 (1-3): : 204 - 210
[6] Histamine, histamine receptors and their role in immune pathology
Jutel, M.
Akdis, M.
Akdis, C. A.
[J]. CLINICAL AND EXPERIMENTAL ALLERGY, 2009, 39 (12) : 1786 - 1800
[7] Pentagalloylglucose down-regulates mast cell surface FcεRI expression in vitro and in vivo
Kageyama-Yahara, Natsuko
Suehiro, Yoko
Maeda, Futoshi
Kageyama, Shun-ichiro
Fukuoka, Junya
Katagiri, Tatsuo
Yamamoto, Takeshi
Kadowaki, Makoto
[J]. FEBS LETTERS, 2010, 584 (01) : 111 - 118
[8] Enzymatic production and biological activities of chitosan oligosaccharides (COS): A review
Kim, SK
Rajapakse, N
[J]. CARBOHYDRATE POLYMERS, 2005, 62 (04) : 357 - 368
[9] Anti-allergic Effects of Phlorotannins on Histamine Release via Binding Inhibition between IgE and FcεRI
Li, Yong
Lee, Sang-Hoon
Le, Quang-To
Kim, Moon-Moo
Kim, Se-Kwon
[J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2008, 56 (24) : 12073 - 12080
[10] Differential regulation of IL-4 expression and degranulation by anti-allergic olopatadine in rat basophilic leukemia (RBL-2H3) cells
Matsubara, M
Masaki, S
Ohmori, K
Karasawa, A
Hasegawa, K
[J]. BIOCHEMICAL PHARMACOLOGY, 2004, 67 (07) : 1315 - 1326

← 1 2 3 →