The human tumor microbiome is composed of tumor type-specific intracellular bacteria

被引：1610

作者：

Nejman, Deborah ^{[1
]}

Livyatan, Ilana ^{[1
,2
]}

Fuks, Garold ^{[3
]}

Gavert, Nancy ^{[1
]}

Zwang, Yaara ^{[1
]}

Geller, Leore T. ^{[1
]}

Rotter-Maskowitz, Aviva ^{[1
]}

Weiser, Roi ^{[4
,5
]}

Mallel, Giuseppe ^{[1
]}

Gigi, Elinor ^{[1
]}

Meltser, Arnon ^{[1
]}

Douglas, Gavin M. ^{[6
]}

Kamer, Iris ^{[7
]}

Gopalakrishnan, Vancheswaran ^{[8
]}

Dadosh, Tali ^{[9
]}

Levin-Zaidman, Smadar ^{[9
]}

Avnet, Sofia ^{[10
]}

Atlan, Tehila ^{[11
]}

Cooper, Zachary A. ^{[12
]}

Arora, Reetakshi ^{[8
]}

Cogdill, Alexandria P. ^{[13
]}

Khan, Md Abdul Wadud ^{[8
]}

Ologun, Gabriel ^{[8
]}

Bussi, Yuval ^{[1
,2
,14
]}

Weinberger, Adina ^{[1
,2
]}

Lotan-Pompan, Maya ^{[1
,2
]}

Golani, Ofra ^{[15
]}

Perry, Gili ^{[16
]}

Rokah, Merav ^{[17
]}

Bahar-Shany, Keren ^{[16
]}

Rozeman, Elisa A. ^{[18
,19
]}

Blank, Christian U. ^{[18
,19
]}

Ronai, Anat ^{[20
]}

Shaoul, Ron ^{[20
]}

Amit, Amnon ^{[21
,22
]}

Dorfman, Tatiana ^{[23
,24
]}

Kremer, Ran ^{[25
]}

Cohen, Zvi R. ^{[5
,26
]}

Harnof, Sagi ^{[5
,27
]}

Siegal, Tali ^{[28
]}

Yehuda-Shnaidman, Einav ^{[29
]}

Gal-Yam, Einav Nili ^{[30
]}

Shapira, Hagit ^{[29
]}

Baldini, Nicola ^{[10
,31
]}

Langille, Morgan G. I. ^{[6
,32
]}

Ben-Nun, Alon ^{[5
,17
]}

Kaufman, Bella ^{[5
,7
]}

Nissan, Aviram ^{[33
]}

Golan, Talia ^{[5
,7
]}

Dadiani, Maya ^{[16
]}

机构：

[1] Weizmann Inst Sci, Dept Mol Cell Biol, Rehovot, Israel

[2] Weizmann Inst Sci, Dept Comp Sci & Appl Math, Rehovot, Israel

[3] Weizmann Inst Sci, Dept Phys Complex Syst, Rehovot, Israel

[4] Tel Aviv Sourasky Med Ctr, Div Surg, Tel Aviv, Israel

[5] Tel Aviv Univ, Sackler Fac Med, Tel Aviv, Israel

[6] Dalhousie Univ, Dept Microbiol & Immunol, Halifax, NS, Canada

[7] Sheba Med Ctr, Inst Oncol, Ramat Gan, Israel

[8] Univ Texas MD Anderson Canc Ctr, Dept Surg Oncol, Houston, TX 77030 USA

[9] Weizmann Inst Sci, Dept Chem Res Support, Rehovot, Israel

[10] Ist Ortoped Rizzoli, IRCCS, Orthopaed Pathophysiol & Regenerat Unit, Bologna, Italy

[11] Jerusalem Coll Technol, Dept Bioinformat, Jerusalem, Israel

[12] AstraZeneca, Oncol R&D, Translat Med, Gaithersburg, MD USA

[13] Univ Texas MD Anderson Canc Ctr, Dept Genom Med, Houston, TX 77030 USA

[14] Weizmann Inst Sci, Dept Biomol Sci, Rehovot, Israel

[15] Weizmann Inst Sci, Dept Life Sci Core Facil, Rehovot, Israel

[16] Sheba Med Ctr, Canc Res Ctr, Ramat Gan, Israel

[17] Sheba Med Ctr, Dept Thorac Surg, Ramat Gan, Israel

[18] Netherlands Canc Inst, Dept Med Oncol, Amsterdam, Netherlands

[19] Netherlands Canc Inst, Div Mol Oncol & Immunol, Amsterdam, Netherlands

[20] Rambam Med Ctr, Pediat Gastroenterol Inst, Haifa, Israel

[21] Technion Israel Inst Technol, Fac Med, Haifa, Israel

[22] Rambam Hlth Care Campus, Dept Obstet & Gynecol, Haifa, Israel

[23] Rambam Hlth Care Campus, Div Gen Surg, Haifa, Israel

[24] Rambam Hlth Care Campus, Ambulatory & Breast Surg Serv, Haifa, Israel

[25] Rambam Hlth Care Campus, Dept Thorac Surg, Haifa, Israel

[26] Sheba Med Ctr, Dept Neurosurg, Ramat Gan, Israel

[27] Beilinson Med Ctr, Rabin Med Ctr, Dept Neurosurg, Petah Tiqwa, Israel

[28] Beilinson Med Ctr, Rabin Med Ctr, Neurooncol Unit, Petah Tiqwa, Israel

[29] Maccabi Healthcare Serv, Megalab, Inst Pathol, Rehovot, Israel

[30] Sheba Med Ctr, Breast Oncol Inst, Ramat Gan, Israel

[31] Univ Bologna, Dept Biomed & Neuromotor Sci, Bologna, Italy

[32] Dalhousie Univ, Dept Pharmacol, Halifax, NS, Canada

[33] Sheba Med Ctr, Dept Surg Oncol Surg C, Ramat Gan, Israel

[34] Sheba Med Ctr, Dept Pathol, Ramat Gan, Israel

[35] Netherlands Canc Inst, Div Mol Oncol & Immunol, Amsterdam, Netherlands

[36] City Hope Med Ctr, Div Thorac Surg, Duarte, CA USA

[37] Open Univ Israel, Dept Math & Comp Sci, Raanana, Israel

来源：

SCIENCE | 2020年 / 368卷 / 6494期

基金：

美国国家卫生研究院; 以色列科学基金会; 欧洲研究理事会; 美国国家科学基金会;

关键词：

INTESTINAL MICROBIOTA; CANCER; CONTAMINATION; LIPOPOLYSACCHARIDE; PROGRESSION; EXTRACTION; DIVERSITY; MULTIPLE; TISSUE;

D O I：

10.1126/science.aay9189

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Bacteria were first detected in human tumors more than 100 years ago, but the characterization of the tumor microbiome has remained challenging because of its low biomass. We undertook a comprehensive analysis of the tumor microbiome, studying 1526 tumors and their adjacent normal tissues across seven cancer types, including breast, lung, ovary, pancreas, melanoma, bone, and brain tumors. We found that each tumor type has a distinct microbiome composition and that breast cancer has a particularly rich and diverse microbiome. The intratumor bacteria are mostly intracellular and are present in both cancer and immune cells. We also noted correlations between intratumor bacteria or their predicted functions with tumor types and subtypes, patients' smoking status, and the response to immunotherapy.

引用

页码：973 / +

页数：66

共 75 条

[1] SNARE priming is essential for maturation of autophagosomes but not for their formation [J].

Abada, Adi ;

Levin-Zaidman, Smadar ;

Porat, Ziv ;

Dadosh, Tali ;

Elazar, Zvulun .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2017, 114 (48) :12749-12754

[2] COMBINATION OF 16S RIBOSOMAL-RNA-TARGETED OLIGONUCLEOTIDE PROBES WITH FLOW-CYTOMETRY FOR ANALYZING MIXED MICROBIAL-POPULATIONS [J].

AMANN, RI ;

BINDER, BJ ;

OLSON, RJ ;

CHISHOLM, SW ;

DEVEREUX, R ;

STAHL, DA .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1990, 56 (06) :1919-1925

[3]

[Anonymous], ZENODO

[4]

[Anonymous], BJOG INT J OBSTET GY

[5]

[Anonymous], 1994, NEW COMPREHENSIVE BI

[6]

[Anonymous], 2020, PICRUST2 IMPROVED EX, DOI DOI 10.1101/672295V1

[7] Bacterial and fungal microflora in surgically removed lung cancer samples [J].

Apostolou, Panagiotis ;

Tsantsaridou, Aggeliki ;

Papasotiriou, Ioannis ;

Toloudi, Maria ;

Chatziioannou, Marina ;

Giamouzis, Gregory .

JOURNAL OF CARDIOTHORACIC SURGERY, 2011, 6

[8] Compact graphical representation of phylogenetic data and metadata with GraPhlAn [J].

Asnicar, Francesco ;

Weingart, George ;

Tickle, Timothy L. ;

Huttenhower, Curtis ;

Segata, Nicola .

PEERJ, 2015, 3

[9]

Baban Chwanrow K, 2010, Bioeng Bugs, V1, P385, DOI 10.4161/bbug.1.6.13146

[10] Distinct Microbial Signatures Associated With Different Breast Cancer Types [J].

Banerjee, Sagarika ;

Tian, Tian ;

Wei, Zhi ;

Shih, Natalie ;

Feldman, Michael D. ;

Peck, Kristen N. ;

DeMichele, Angela M. ;

Alwine, James C. ;

Robertson, Erle S. .

FRONTIERS IN MICROBIOLOGY, 2018, 9

← 1 2 3 4 5 6 7 8 →