Comparative study of the effects of phosphatidylcholine rich in DHA and EPA on Alzheimer's disease and the possible mechanisms in CHO-APP/PS1 cells and SAMP8 mice

被引：69

作者：

Che, Hongxia ^{[1
]}

Zhou, Miaomiao ^{[1
]}

Zhang, Tiantian ^{[1
]}

Zhang, Lingyu ^{[1
]}

Ding, Lin ^{[1
]}

Yanagita, Teruyoshi ^{[2
]}

Xu, Jie ^{[1
]}

Xue, Changhu ^{[1
,3
]}

Wang, Yuming ^{[1
,3
]}

机构：

[1] Ocean Univ China, Coll Food Sci & Engn, Qingdao 266003, Shandong, Peoples R China

[2] Nishikyushu Univ, Dept Hlth & Nutr Sci, Kanzaki, Japan

[3] Qingdao Natl Lab Marine Sci & Technol, Lab Marine Drugs & Biol Prod, Qingdao 266237, Peoples R China

来源：

FOOD & FUNCTION | 2018年 / 9卷 / 01期

基金：

中国国家自然科学基金;

关键词：

POLYUNSATURATED FATTY-ACIDS; PROTEIN A-BETA; OXIDATIVE STRESS; BRAIN; PHOSPHOLIPIDS; PLASMALOGEN; OMEGA-3-FATTY-ACIDS; ETHANOLAMINE;

D O I：

10.1039/c7fo01342f

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Metabolic stress induced by a high-fat (HF) diet leads to cognitive dysfunction and aging. In the present study, Chinese hamster ovary cells stably transfected with amyloid precursor protein (APP) and presenilin 1 (PS1) (CHO-APP/PS1 cells) and SAMP8 mice fed with an HF diet were used to study the effects of docosahexaenoic acid (DHA)-enriched phosphatidylcholine (DHA-PC) and eicosapentaenoic acid (EPA)-enriched phosphatidylcholine (EPA-PC) on Alzheimer's disease (AD) and the possible mechanisms involved in these effects. Behavior test results indicated that DHA-PC exerted better effects than EPA-PC on improving memory and cognitive deficiency. Further analysis showed that DHA-PC and EPA-PC could significantly decrease beta-amyloid (A beta) concentrations in CHO-APP/PS1 cells and SAMP8 mice by inhibiting APP, PS1, and BACE1 expression. Moreover, both DHA-PC and EPA-PC can increase the activities of the antioxidant index, including SOD, T-AOC, GSH, and GSH-PX, and inhibit levels of MDA, NO, and NOS. In addition, the expressions of inflammatory factors (TNF-alpha IL-1 beta) and apoptosis were significantly suppressed via improving the ratio of Bcl-2/Bax and decreasing the expression of pro-apoptosis factors. Interestingly, only DHA-PC could improve the expression of neurotrophic factors, including BDNF, synaptophysin, and growth associated protein 43. DHA-PC and EPA-PC could ameliorate memory and cognitive function of HF diet-fed SAMP8 mice via inhibiting A beta generation, suppressing oxidative stress and apoptosis, down-regulating inflammatory response, and improving neurotrophic activity. Therefore, DHA-PC and EPA-PC may be applied as food supplements and/or functional ingredients to relieve neurodegenerative disease.

引用

页码：643 / 654

页数：12

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