In vivo tumor suppression by adenovirus-mediated interferon α2b gene delivery

被引：25

作者：

Ahmed, CMI ^{[1
]}

Sugarman, BJ ^{[1
]}

Johnson, DE ^{[1
]}

Bookstein, RE ^{[1
]}

Saha, DP ^{[1
]}

Nagabhushan, TL ^{[1
]}

Wills, KN ^{[1
]}

机构：

[1] Canji Inc, San Diego, CA 92121 USA

来源：

HUMAN GENE THERAPY | 1999年 / 10卷 / 01期

关键词：

D O I：

10.1089/10430349950019200

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A replication-deficient adenovirus encoding human interferon alpha 2b, driven by the human cytomegalovirus (CMV) promoter, was constructed and characterized. This construct was used to infect human cells derived from different types of cancer, The production of protein and its secretion into the culture medium were tested by Western blotting and immunoassay, Inhibition of cell proliferation and antiviral activity, two of the most important biological activities of interferon, were observed with this construct. PC-3 cells, derived from human prostatic cancer, or Hep3B cells, derived from human hepatocellular carcinoma, were injected subcutaneously to generate and establish in vivo tumors in athymic nude mice. Intratumoral injection with the recombinant adenovirus expressing interferon alpha 2b resulted in complete regression of tumor growth. Our results demonstrate that interferon gene delivery using recombinant adenoviral vectors may be a useful approach to treat a variety of cancers.

引用

页码：77 / 84

页数：8

共 22 条

[1] NATURAL HUMAN INTERFERON-ALPHA-2 IS O-GLYCOSYLATED [J].