A simplified cytomegalovirus pp65 antigenemia assay procedure

被引:2
作者
Gratacap-Cavallier, B [1 ]
Bonadona, A
Berthier, R
Brambilla, E
Seigneurin, JM
Lorimier, P
机构
[1] CHU, Virol Lab, F-38043 Grenoble 09, France
[2] CHU, Serv Reanimat Med, F-38043 Grenoble, France
[3] CHU, Serv Reanimat Med, F-38043 Grenoble, France
[4] CHU Grenoble, Pathol Cellulaire Lab, F-38043 Grenoble, France
关键词
cytomegalovirus; pp65; antigenemia; rapid; assay;
D O I
10.1016/S1386-6532(03)00075-1
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
A simplified cytomegalovirus (CMV) pp65 antigenemia assay using a one-step erythrocyte lysis, fixation and permeabilization process was compared with a standard protocol, the CMV CINAkit (Argene Biosoft) assay. The results were comparable, both quantitatively and qualitatively. The new method saves time. It also provides flexibility because the cell suspension can be stored so that test completion can be deferred if so desired. (C) 2003 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:317 / 322
页数:6
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