Development of eight polymorphic microsatellite markers in the Black and Rufous sengi, Rhynchocyon petersi

被引:2
作者
Sabuni, Christopher A. [1 ]
Van Houtte, Natalie [2 ]
Maganga, Samwel L. S. [3 ]
Makundi, Rhodes H. [1 ]
Leirs, Herwig [2 ]
de Bellocq, Joelle Gouy [2 ,4 ]
机构
[1] Sokoine Univ Agr, Ctr Pest Management, Morogoro, Tanzania
[2] Univ Antwerp, Evolutionary Ecol Grp, B-2020 Antwerp, Belgium
[3] Sokoine Univ Agr, Dept Wildlife Management, Morogoro, Tanzania
[4] Acad Sci Czech Republ, Res Facil Studenec, Inst Vertebrate Biol, Brno, Czech Republic
关键词
Rhynchocyon petersi; Vulnerable afrotherian; Microsatellites; 454; Sequencing;
D O I
10.1007/s12686-014-0330-5
中图分类号
X176 [生物多样性保护];
学科分类号
090705 ;
摘要
The Black and Rufous sengi, Ryhnchocyon petersi, is endemic to a limited range in East Africa. We report the development of eight polymorphic microsatellites using next generation sequencing technology. Eighteen individuals from Zaraninge forest (Saadani National Park, Tanzania) were genotyped. The number of alleles per locus ranged from 2 to 6, while the observed and expected heterozygosities varied from 0.17 to 0.82 and from 0.25 to 0.81, respectively. No locus deviated from Hardy-Weinberg equilibrium. These microsatellite markers will be useful tools to study the effect of habitat fragmentation on the population genetic structure of R. petersi.
引用
收藏
页码:193 / 195
页数:3
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