The nanoscale organization of Nipah virus matrix protein revealed by super-resolution microscopy

被引：2

作者：

Liu, Qian T. ^{[1
,2
]}

Wang, Qian ^{[3
]}

Zhang, Youchang ^{[1
,2
]}

Kliemke, Vicky ^{[3
]}

Liu, Qian ^{[3
]}

Chou, Keng C. ^{[1
,2
]}

机构：

[1] Univ British Columbia, Dept Chem, Vancouver, BC, Canada

[2] Univ British Columbia, Life Sci Inst, Vancouver, BC, Canada

[3] McGill Univ, Inst Parasitol, Ste Anne De Bellevue, PQ, Canada

来源：

BIOPHYSICAL JOURNAL | 2022年 / 121卷 / 12期

基金：

加拿大自然科学与工程研究理事会;

关键词：

LOCALIZATION;

D O I：

10.1016/j.bpj.2022.05.026

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

The matrix proteins (M) of many enveloped RNA viruses mediate virus assembly and budding. However, it remains poorly understood how M are involved in virus budding and how they interact with envelope proteins. Here, we show that the expression level of Nipah (NiV) M in particles produced by the host cells deviates from a gamma distribution and does not reflect that of the host cells, indicating assembly of the NiV-M in the process. Our data reveal that NiV-M affects the circularity of the particles while the NiV envelope proteins do not. The organization of NiV envelope proteins on the membrane of the particles is similar to those that do not express NiV-M, suggesting that NiV-M does not directly interact with the envelope proteins during assembly and budding.

引用

页码：2290 / 2296

页数：7

共 42 条

[1] Structure and assembly of a paramyxovirus matrix protein [J].

Battisti, Anthony J. ;

Meng, Geng ;

Winkler, Dennis C. ;

McGinnes, Lori W. ;

Plevka, Pavel ;

Steven, Alasdair C. ;

Morrison, Trudy G. ;

Rossmann, Michael G. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2012, 109 (35) :13996-14000

[2] Imaging intracellular fluorescent proteins at nanometer resolution [J].

Betzig, Eric ;

Patterson, George H. ;

Sougrat, Rachid ;

Lindwasser, O. Wolf ;

Olenych, Scott ;

Bonifacino, Juan S. ;

Davidson, Michael W. ;

Lippincott-Schwartz, Jennifer ;

Hess, Harald F. .

SCIENCE, 2006, 313 (5793) :1642-1645

[3]

Chazotte Brad, 2011, Cold Spring Harb Protoc, V2011, DOI 10.1101/pdb.prot5623

[4] Palmitoylation Contributes to Membrane Curvature in Influenza A Virus Assembly and Hemagglutinin-Mediated Membrane Fusion [J].

Chlanda, Petr ;

Mekhedov, Elena ;

Waters, Hang ;

Sodt, Alexander ;

Schwartz, Cindi ;

Nair, Vinod ;

Blank, Paul S. ;

Zimmerberg, Joshua .

JOURNAL OF VIROLOGY, 2017, 91 (21)

[5]

Chua K B, 2007, Med J Malaysia, V62, P139

[6] Mutation of YMYL in the Nipah virus matrix protein abrogates budding and alters subcellular localization [J].

Ciancanelli, Michael J. ;

Basler, Christopher F. .

JOURNAL OF VIROLOGY, 2006, 80 (24) :12070-12078

[7]

Dempsey GT, 2011, NAT METHODS, V8, P1027, DOI [10.1038/NMETH.1768, 10.1038/nmeth.1768]

[8] Hendra and Nipah viruses: different and dangerous [J].

Eaton, BT ;

Broder, CC ;

Middleton, D ;

Wang, LF .

NATURE REVIEWS MICROBIOLOGY, 2006, 4 (01) :23-35

[9] Localization of the Epstein-Barr virus protein LMP 1 to exosomes [J].

Flanagan, J ;

Middeldorp, J ;

Sculley, T .

JOURNAL OF GENERAL VIROLOGY, 2003, 84 :1871-1879

[10] Linking stochastic dynamics to population distribution: An analytical framework of gene expression [J].

Friedman, Nir ;

Cai, Long ;

Xie, X. Sunney .

PHYSICAL REVIEW LETTERS, 2006, 97 (16)

← 1 2 3 4 5 →