Affinity ligands for industrial protein purification

被引：26

作者：

Linhult, M ^{[1
]}

Gülich, S

Hober, S

机构：

[1] Albanova Univ Ctr, Dept Biotechnol, Royal Inst Technol, SE-10691 Stockholm, Sweden

[2] Yale Univ, Dept Mol Biophys & Biochem, New Haven, CT 06520 USA

来源：

PROTEIN AND PEPTIDE LETTERS | 2005年 / 12卷 / 04期

关键词：

Affinity chromatography; cleaning-in-place (CIP); deamidation; protein A; protein G; protein engineering; stabilization;

D O I：

10.2174/0929866053765662

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Significant efforts are put into the design of large-scale purification processes of proteins due to great demands regarding cost efficiency and safety. In order to design an effective purification scheme the unit operations need to be reduced to a minimum. In this review we are discussing proteinaceous ligands as well as small synthetic mimics for use in affinity chromatography for large-scale applications. Different advantages as well as drawbacks of the two approaches are outlined.

引用

页码：305 / 310

页数：6

共 41 条

[1] PROPERTIES OF A SINGLE-CHAIN ANTIBODY CONTAINING DIFFERENT LINKER PEPTIDES [J].

ALFTHAN, K ;

TAKKINEN, K ;

SIZMANN, D ;

SODERLUND, H ;

TERRI, TT .

PROTEIN ENGINEERING, 1995, 8 (07) :725-731

[2] Design of the linkers which effectively separate domains of a bifunctional fusion protein [J].

Arai, R ;

Ueda, H ;

Kitayama, A ;

Kamiya, N ;

Nagamune, T .

PROTEIN ENGINEERING, 2001, 14 (08) :529-532

[3] Development of a cleaning in place protocol and repetitive application of Escherichia coli homogenate on STREAMLINE™ Q XL [J].

Asplund, M ;

Ramberg, M ;

Johansson, BL .

PROCESS BIOCHEMISTRY, 2000, 35 (10) :1111-1118

[4] SYSTEMATIC DEVELOPMENT AND VALIDATION OF SANITIZATION PROTOCOLS FOR A CHROMATOGRAPHIC SYSTEM DESIGNED FOR BIOTHERAPEUTICS PURIFICATION [J].

BURGOYNE, RF ;

PRIEST, MC ;

ROCHE, KL ;

VELLA, G .

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 1993, 11 (11-12) :1317-1325

[5] Affinity chromatography in the industrial purification of plasma proteins for therapeutic use [J].

Burnouf, T ;

Radosevich, M .

JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS, 2001, 49 (1-3) :575-586

[6]

Fassina G, 1998, J MOL RECOGNIT, V11, P128, DOI 10.1002/(SICI)1099-1352(199812)11:1/6<128::AID-JMR408>3.0.CO

[7]

2-8

[8]

GEIGER T, 1987, J BIOL CHEM, V262, P785

[9] An analysis of protein domain linkers: their classification and role in protein folding [J].

George, RA ;

Heringa, J .

PROTEIN ENGINEERING, 2002, 15 (11) :871-879

[10] COMPOSITE AFFINITY SORBENTS AND THEIR CLEANING IN PLACE [J].

GIROT, P ;

MOROUX, Y ;

DUTEIL, XP ;

NGUYEN, C ;

BOSCHETTI, E .

JOURNAL OF CHROMATOGRAPHY, 1990, 510 :213-223

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