Expression of the miR-9-5p, miR-125b-5p and its target gene NFKB1 and TRAF6 in childhood-onset systemic lupus erythematosus (cSLE)

被引：7

作者：

Nascimento, Denise de Queiroga ^{[1
,2
]}

Fonseca Gomes da Silva, Isaura Isabelle ^{[1
,2
]}

Dantas Lima, Camilla Albertina ^{[2
,3
]}

Cavalcanti, Andre de Souza ^{[4
]}

Roberti, Luciana Rodrigues ^{[5
]}

de Paula Queiroz, Rosane Gomes ^{[5
]}

Leme Ferriani, Virginia Paes ^{[5
]}

Crovella, Sergio ^{[6
]}

de Carvalho, Luciana Martins ^{[5
]}

Sandrin-Garcia, Paula ^{[1
,2
]}

机构：

[1] Univ Fed Pernambuco, Dept Genet, PostGrad Program Genet, Recife, PE, Brazil

[2] Lab Immunopathol Keizo Asami, Recife, PE, Brazil

[3] Univ Fed Pernambuco, Dept Oceanog, Recife, PE, Brazil

[4] Univ Fed Pernambuco, Div Pediat Rheumatol, Clin Hosp, Recife, PE, Brazil

[5] Univ Sao Paulo, Clin Hosp, Div Pediat Rheumatol, Ribeirao Preto Med Sch, Sao Paulo, Brazil

[6] Qatar Univ, Coll Arts & Sci, Dept Biol & Environm Sci, Doha, Qatar

来源：

AUTOIMMUNITY | 2022年 / 55卷 / 08期

关键词：

cSLE; miR-125b; miR-9; inflammation; gene expression; MICRORNAS; DISEASE;

D O I：

10.1080/08916934.2022.2128781

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Childhood- onset systemic lupus erythematosus (cSLE) is a multisystem inflammatory disease that can lead to severe clinical conditions resulting in early comorbidities. Several genetic, environmental, and immunological factors are known to influence the onset of the disease. MiRNAs have been already considered as potential actors involved in the development and activity of the SLE. Thus, understanding the behavior of these regulators can contribute to clarify the inflammatory process affecting SLE patients. Among miRNAs, miR-125b-5p and miR-9-5p targeting NFKB1 and TRAF6 genes can be involved in the etio-pathogenesis of the disease by modulating inflammation. In this study we evaluated miR-9-5p and miR-125b-5p expression and its target genes NFKB1 and TRAF6 in peripheral blood samples (PBMC) from the 35 cSLE patients and 35 healthy controls. MiRNAs and gene target expression have been evaluated by using RT-PCR with specific TaqMan (R) probes. Both miR-9-5p [Fold Change (FC) = -2.21; p = 0.002] and miR-125b-5p (FC= -3.30; p < 0.0001) and NFKB1 (FC = -1.84; p < 0.001) were downregulated in cSLE patients, while TRAF6 was upregulated (FC = 1.80; p = 0.006) in cSLE patients when compared to controls. A significant correlation was found between miR-125b-5p and its target gene NFKB1 [Spearman (r) = 0.47; p = 0.023]. Our results showed miR-125b-5p and miR-9-5p differential expression in cSLE patients, possibly contributing to better understanding the role of these regulators in cSLE development and disease pathogenesis.

引用

页码：515 / 519

页数：5