Detection of Plasmodium Sporozoites in Anopheles Mosquitoes using an Enzyme-linked Immunosorbent Assay

被引：3

作者：

Kumpitak, Chalermpon ^{[1
]}

Nguitragool, Wang ^{[2
]}

Cui, Liwang ^{[3
]}

Sattabongkot, Jetsumon ^{[1
]}

Bantuchai, Sirasate ^{[1
]}

机构：

[1] Mahidol Univ, Fac Trop Med, Mahidol Vivax Res Unit, Salaya, Nakhon Pathom, Thailand

[2] Mahidol Univ, Fac Trop Med, Dept Mol Trop Med & Genet, Salaya, Nakhon Pathom, Thailand

[3] Univ S Florida, Morsani Coll Med, Dept Internal Med, Tampa, FL 33620 USA

来源：

JOVE-JOURNAL OF VISUALIZED EXPERIMENTS | 2021年 / 175期

关键词：

POLYMERASE-CHAIN-REACTION; VIVAX SPOROZOITES; MALARIA; IDENTIFICATION; FALCIPARUM; CULICIDAE; DIPTERA;

D O I：

10.3791/63158

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Plasmodium sporozoites are the infective stage of malaria parasites that infect humans. The sporozoites residing in the salivary glands of female Anopheles mosquitoes are transmitted to humans via mosquito bites during blood feeding. The presence of sporozoites in the mosquito salivary glands thus defines mosquito infectiousness. To determine whether an Anopheles mosquito carries Plasmodium sporozoites, the enzyme-linked immunosorbent assay (ELISA) method has been the standard tool to detect the Plasmodium circumsporozoite protein (CSP), the major surface protein of the sporozoites. In this method, the head along with the thorax of each mosquito is separated from the abdomen, homogenized, and subjected to a sandwich ELISA to detect the presence of CSP specific to Plasmodium falciparum and each of the two subtypes, VK210 and VK247, of Plasmodium vivax.This method has been used to study malaria transmission, including the seasonal dynamics of mosquito infection and the species of the major malaria vectors in the study sites.

引用

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