SV40 associated miRNAs are not detectable in mesotheliomas

被引：15

作者：

Gee, G. V. ^{[1
]}

Stanifer, M. L. ^{[2
,3
]}

Christensen, B. C. ^{[1
,4
]}

Atwood, W. J. ^{[3
]}

Ugolini, D. ^{[5
]}

Bonassi, S. ^{[6
,7
]}

Resnick, M. B. ^{[1
]}

Nelson, H. H. ^{[8
]}

Marsit, C. J. ^{[1
]}

Kelsey, K. T. ^{[1
,4
]}

机构：

[1] Brown Univ, Dept Pathol & Lab Med, Providence, RI 02912 USA

[2] Brown Univ, Grad Program Mol Biol Cell Biol & Biochem, Providence, RI 02912 USA

[3] Brown Univ, Dept Mol Biol Cell Biol & Biochem, Providence, RI USA

[4] Brown Univ, Dept Community Hlth, Providence, RI 02912 USA

[5] Univ Genoa, Dept Oncol Biol & Genet, Genoa, Italy

[6] Univ Minnesota, Div Epidemiol, Mason Canc Ctr, Minneapolis, MN 55455 USA

[7] Natl Inst Canc Res, Dept Environm Epidemiol, Unit Clin & Mol Epidemiol, Rome, Italy

[8] Univ Minnesota, Sch Publ Hlth, Div Epidemiol & Community Hlth, Mason Canc Ctr, Minneapolis, MN USA

来源：

BRITISH JOURNAL OF CANCER | 2010年 / 103卷 / 06期

关键词：

mesothelioma; microRNA; SV40; CELLS; VIRUS; TRANSFORMATION; DNA; SEQUENCES; MICRORNAS;

D O I：

10.1038/sj.bjc.6605848

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

BACKGROUND: Simian virus-40 (SV40) is a DNA tumour virus that was introduced into the human population with contaminated poliovirus vaccine, and its role in mesothelioma is widely debated. PCR based testing has been called into question, as false positives can be because of cross-reactivity with related viruses, or to laboratory contamination. The Institute of Medicine has recommended the development of more sensitive and specific tests to resolve this controversy. METHODS: We have characterized highly sensitive RT-PCR based assays that are specific for SV40-encoded microRNAs (miRNAs), as an alternative to current testing methods. RESULTS: Using this sensitive and specific detection method, we were unable to identify SV40 miRNA expression in human malignant pleural mesothelioma (MM) samples. CONCLUSION: Our work indicates that SV40 miRNAs are not likely to contribute to mesothelioma tumourogenesis, but highlights the value of this approach when compared with the relatively unspecific current testing methods. British Journal of Cancer (2010) 103, 885-888. doi:10.1038/sj.bjc.6605848 www.bjcancer.com Published online 17 August 2010 (C) 2010 Cancer Research UK

引用

页码：885 / 888

页数：4

共 50 条

[41] A fail-safe system to prevent oncogenesis by senescence is targeted by SV40 small T antigen [J].

Oshikawa, Kiyotaka ;

Matsumoto, Masaki ;

Kodama, Manabu ;

Shimizu, Hideyuki ;

Nakayama, Keiichi I. .

ONCOGENE, 2020, 39 (10) :2170-2186

[42] SV40 can be reproducibly detected in paraffin-embedded mesothelioma samples [J].

Cristaudo, A ;

Powers, A ;

Vivaldi, A ;

Foddis, R ;

Guglielmi, G ;

Gattini, V ;

Buselli, R ;

Sensales, G ;

Ciancia, E ;

Ottenga, F .

ANTICANCER RESEARCH, 2000, 20 (2A) :895-898

[43] Prevalence of polyomaviruses BK, JC, SV40, KI, and WU in non-malignant tonsil specimens [J].

Astegiano, S. ;

Terlizzi, M. E. ;

Elia, M. ;

Cavallo, G. P. ;

Costa, C. ;

Cavallo, R. ;

Bergallo, M. .

MINERVA MEDICA, 2010, 101 (06) :385-389

[44] IMMORTALIZATION OF IMMATURE AND MATURE MOUSE ASTROCYTES WITH SV40 T-ANTIGEN [J].

FRISA, PS ;

GOODMAN, MN ;

SMITH, GM ;

SILVER, J ;

JACOBBERGER, JW .

JOURNAL OF NEUROSCIENCE RESEARCH, 1994, 39 (01) :47-56

[45] SV40 in human tumors: New documents shed light on the apparent controversy [J].

MacLachlan, DS .

ANTICANCER RESEARCH, 2002, 22 (6B) :3495-3499

[46] Study of SV40 large T antigen nucleotide specificity for DNA unwinding [J].

Wang, Damian ;

Alvarez-Cabrera, Ana Lucia ;

Chen, Xiaojiang S. .

VIROLOGY JOURNAL, 2017, 14

[47] Fanconi Anemia Patients Are More Susceptible to Infection with Tumor Virus SV40 [J].

Comar, Manola ;

De Rocco, Daniela ;

Cappelli, Enrico ;

Zanotta, Nunzia ;

Bottega, Roberta ;

Svahn, Johanna ;

Farruggia, Piero ;

Misuraca, Aldo ;

Corsolini, Fabio ;

Dufour, Carlo ;

Savoia, Anna .

PLOS ONE, 2013, 8 (11)

[48] PERSISTENCE OF THE SV40 EARLY REGION WITHOUT EXPRESSION IN PERMISSIVE SIMIAN CELLS [J].

ONEILL, FJ ;

XU, XL ;

GAO, Y ;

ROSENFELD, M ;

JENKINS, F ;

CARNEY, H .

VIRUS RESEARCH, 1994, 34 (03) :237-263

[49] Sera from Patients with Malignant Pleural Mesothelioma Tested Positive for IgG Antibodies against SV40 Large T Antigen: The Viral Oncoprotein [J].

Mazzoni, Elisa ;

Bononi, Ilaria ;

Rotondo, John Charles ;

Mazziotta, Chiara ;

Libener, Roberta ;

Guaschino, Roberto ;

Gafa, Roberta ;

Lanza, Giovanni ;

Martini, Fernanda ;

Tognon, Mauro .

JOURNAL OF ONCOLOGY, 2022, 2022

[50] Does Simian Virus 40 (SV40) Have a Role in UK Malignant Pleural Mesothelioma? No Role is Identified in a Sensitive RNA In Situ Hybridization Study on Potentially Affected Birth Cohorts [J].

Alchami, Fouad S. ;

Attanoos, Richard L. ;

Gibbs, Allen ;

Morgan, Fiona ;

Jasani, Bharat .

APPLIED IMMUNOHISTOCHEMISTRY & MOLECULAR MORPHOLOGY, 2020, 28 (06) :444-447

← 1 2 3 4 5 →