SV40 associated miRNAs are not detectable in mesotheliomas

被引：15
作者：
Gee, G. V. [1 ]
Stanifer, M. L. [2 ,3 ]
Christensen, B. C. [1 ,4 ]
Atwood, W. J. [3 ]
Ugolini, D. [5 ]
Bonassi, S. [6 ,7 ]
Resnick, M. B. [1 ]
Nelson, H. H. [8 ]
Marsit, C. J. [1 ]
Kelsey, K. T. [1 ,4 ]
机构：
[1] Brown Univ, Dept Pathol & Lab Med, Providence, RI 02912 USA
[2] Brown Univ, Grad Program Mol Biol Cell Biol & Biochem, Providence, RI 02912 USA
[3] Brown Univ, Dept Mol Biol Cell Biol & Biochem, Providence, RI USA
[4] Brown Univ, Dept Community Hlth, Providence, RI 02912 USA
[5] Univ Genoa, Dept Oncol Biol & Genet, Genoa, Italy
[6] Univ Minnesota, Div Epidemiol, Mason Canc Ctr, Minneapolis, MN 55455 USA
[7] Natl Inst Canc Res, Dept Environm Epidemiol, Unit Clin & Mol Epidemiol, Rome, Italy
[8] Univ Minnesota, Sch Publ Hlth, Div Epidemiol & Community Hlth, Mason Canc Ctr, Minneapolis, MN USA
来源：
BRITISH JOURNAL OF CANCER | 2010年 / 103卷 / 06期
关键词：
mesothelioma; microRNA; SV40; CELLS; VIRUS; TRANSFORMATION; DNA; SEQUENCES; MICRORNAS;
D O I：
10.1038/sj.bjc.6605848
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
BACKGROUND: Simian virus-40 (SV40) is a DNA tumour virus that was introduced into the human population with contaminated poliovirus vaccine, and its role in mesothelioma is widely debated. PCR based testing has been called into question, as false positives can be because of cross-reactivity with related viruses, or to laboratory contamination. The Institute of Medicine has recommended the development of more sensitive and specific tests to resolve this controversy. METHODS: We have characterized highly sensitive RT-PCR based assays that are specific for SV40-encoded microRNAs (miRNAs), as an alternative to current testing methods. RESULTS: Using this sensitive and specific detection method, we were unable to identify SV40 miRNA expression in human malignant pleural mesothelioma (MM) samples. CONCLUSION: Our work indicates that SV40 miRNAs are not likely to contribute to mesothelioma tumourogenesis, but highlights the value of this approach when compared with the relatively unspecific current testing methods. British Journal of Cancer (2010) 103, 885-888. doi:10.1038/sj.bjc.6605848 www.bjcancer.com Published online 17 August 2010 (C) 2010 Cancer Research UK
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收藏
页码：885 / 888
页数：4
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