Integrinβ 1-mediated signaling is involved in transforming growth factor-β 2-promoted migration in human lens epithelial cells

被引:0
作者
Yao, Ke [1 ]
Tan, Jian
Ye, PanPan
Wang, KaiJun
Xu, Wen
ShenTu, XingChao
Tang, XiaJing
机构
[1] Zhejiang Univ, Coll Med, Ctr Eye, Affiliated Hosp 2, Hangzhou 310009, Peoples R China
[2] Fudan Univ, Dept Ophthalmol, Eye & ENT Hosp, Shanghai 200433, Peoples R China
来源
MOLECULAR VISION | 2007年 / 13卷 / 197期
关键词
D O I
暂无
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Purpose: Transforming growth factor-beta 2 (TGF-beta 2) is a potent growth inhibitor and apoptosis inducer. However, typically TGF-beta 2 loses its growth-inhibitory and apoptosis-inducing effects but stimulates the migratory capacity of epithelial cells. In this study, we investigate the possible involvement of integrin and integrin-mediated signaling in TGF-beta 2-promoted migration and adhesion in human lens epithelial cells. Methods: A human lens epithelial cell line (HLE B-3) was treated with 100 pg/ml TGF-beta 2 for 6 h, 12 h, and 24 h. In vitro wound healing assay and cell adhesion assay were performed to detect the effect of TGF-beta 2 on HLEC adhesion and migration. Integrin beta 1 expression changes in HLECs during the treatment of TGF-beta 2 were detected in protein levels and mRNA levels using confocal microscopy, flow cytometric analysis, and real time quantitative reverse transcription polymerase chain reaction (RT-PCR). Focal adhesion kinase (FAK) activity was examined by FAK phosphorylation and total tyrosine phosphorylation during treatment with TGF-beta 2 in HLEC. Production of endogenous TGF-beta 2 was measured by ELISA assay. Results: In this study, we found that TGF-beta 2 significantly stimulated cell adhesion and migration in HLECs. By immunofluorescence staining and western blotting, we observed that TGF-beta 2 markedly enhanced the expression of integrin beta 1 and the Tyr-phosphorylation of focal adhesion kinase (FAK). Real time quantitative RT-PCR also showed the mRNA level of integrin beta 1 was upregulated. Neutralizing anti-integrin beta 1 monoclonal antibody significantly (p < 0.05) inhibited TGF-beta 2-promoted HLEC adhesion and migration. Conclusions: TGF-beta 2 promoted HLEC adhesion and migration in vitro. Integrin beta 1 and integrin-mediated signaling are necessary for TGF-beta 2-promoted adhesion and migration in human lens epithelial cells.
引用
收藏
页码:1769 / 1776
页数:8
相关论文
共 39 条
  • [1] ANDLEY UP, 1994, INVEST OPHTH VIS SCI, V35, P3094
  • [2] POSTERIOR CAPSULE OPACIFICATION
    APPLE, DJ
    SOLOMON, KD
    TETZ, MR
    ASSIA, EI
    HOLLAND, EY
    LEGLER, UFC
    TSAI, JC
    CASTANEDA, VE
    HOGGATT, JP
    KOSTICK, AMP
    [J]. SURVEY OF OPHTHALMOLOGY, 1992, 37 (02) : 73 - 116
  • [3] Duel nature of TGF-β signaling:: tumor suppressor vs. tumor promoter
    Bachman, KE
    Park, BH
    [J]. CURRENT OPINION IN ONCOLOGY, 2005, 17 (01) : 49 - 54
  • [4] Posterior capsule opacification and anterior capsule opacification
    Bertelmann, Eckart
    Kojetinsky, Corina
    [J]. CURRENT OPINION IN OPHTHALMOLOGY, 2001, 12 (01) : 35 - 40
  • [5] Functional structure and composition of the extracellular matrix
    Bosman, FT
    Stamenkovic, I
    [J]. JOURNAL OF PATHOLOGY, 2003, 200 (04) : 423 - 428
  • [6] BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
  • [7] Cary LA, 1996, J CELL SCI, V109, P1787
  • [8] CHEN YP, 1994, J BIOL CHEM, V269, P18307
  • [9] Clark D S, 2000, Curr Opin Ophthalmol, V11, P56, DOI 10.1097/00055735-200002000-00009
  • [10] Bi-directional signal transduction by integrin receptors
    Coppolino, MG
    Dedhar, S
    [J]. INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY, 2000, 32 (02) : 171 - 188