Mathematical modeling and experimental results of a sandwich-type amperometric biosensor

被引:31
作者
Ricardo Romero, Marcelo [1 ]
Baruzzi, Ana M. [1 ]
Garay, Fernando [1 ]
机构
[1] UNC, INFIQC, Dpto Fis Quim, Fac Ciencias Quim, RA-5000 Cordoba, Argentina
关键词
Ping-pong; Amperometric sensor; Finite difference; Sandwich-type biosensor; Lactate; Hydrogel; Oxidase; CHARGE NEUTRALIZATION PROCESS; ROTATING-DISC ELECTRODE; GLUCOSE-OXIDASE; ENZYME ELECTRODE; ELECTROCHEMICAL OXIDATION; CONCENTRATION GRADIENTS; PLATINUM-ELECTRODES; HYDROGEN-PEROXIDE; PART; SIMULATION;
D O I
10.1016/j.snb.2011.12.079
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
A comprehensive numerical treatment of the diffusion and reaction within a sandwich-type amperometric biosensor is presented. The model considers that the enzyme reacts according to a ping-pong mechanism and that it is entrapped into a so-called enzymatic matrix placed between two diffusion membranes. It is found that the concentrations of reagents and products within the sensor are difficult of being compared to those of the bulk. In this regard, the use of approximate analytical solutions would involve errors in the analysis of kinetic parameters corresponding to this kind of biosensors. Provided the mediator species are in high concentration or diffuse much faster than the substrate, the response time of a biosensor of this kind would be determined by the diffusion of the substrate though the external membrane. In this sense, those systems with immobilized mediators, in which diffusion of electrons or holes is assumed for the charge transport process, could be also described by this model. Thus, the thickness and the permeability to the analyte of the external membrane are critical parameters for improving the response time of a sandwich-type biosensor. The simulated curves are compared with experimental profiles corresponding to a lactate amperometric biosensor obtaining consistent results. In a future publication a non-linear fitting algorithm will be combined to the model for the extraction of kinetic and/or geometric parameters. (C) 2012 Elsevier B.V. All rights reserved.
引用
收藏
页码:284 / 291
页数:8
相关论文
共 50 条
[31]   Zinc(II) Containing γ-Keggin Sandwich-Type Silicotungstate: Synthesis in Organic Media and Oxidation Catalysis [J].
Kikukawa, Yuji ;
Yamaguchi, Kazuya ;
Mizuno, Noritaka .
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2010, 49 (35) :6096-6100
[32]   Sandwich-type aptasensor employing modified aptamers and enzyme-DNA binding protein conjugates [J].
Gülsen Betül Aktas ;
Vasso Skouridou ;
Lluis Masip .
Analytical and Bioanalytical Chemistry, 2019, 411 :3581-3589
[33]   Multifunctional catalyst based on sandwich-type polyoxotungstate and MIL-101 for liquid phase oxidations [J].
Balula, Salete S. ;
Granadeiro, Carlos M. ;
Barbosa, Andre D. S. ;
Santos, Isabel C. M. S. ;
Cunha-Silva, Luis .
CATALYSIS TODAY, 2013, 210 :142-148
[34]   SANDWICH ENZYME MEMBRANES FOR AMPEROMETRIC MULTI-BIOSENSOR APPLICATIONS - IMPROVEMENT OF LINEARITY AND REDUCTION OF CHEMICAL CROSS-TALK [J].
POPP, J ;
SILBER, A ;
BRAUCHLE, C ;
HAMPP, N .
BIOSENSORS & BIOELECTRONICS, 1995, 10 (3-4) :243-249
[35]   Two Keggin sandwich-type tungstophosphates modified by open-chain carboxyethyltin groups and transition metals [J].
Zhang, Bai ;
Zhang, Lan-Cui ;
Zhang, Ya-Jun ;
Su, Fang ;
You, Wan-Sheng ;
Zhu, Zai-Ming .
RSC ADVANCES, 2015, 5 (59) :47319-47325
[36]   Sandwich-Type Enzymatic Fuel Cell Based on a New Electro-Conductive Material - Ion Jelly [J].
Carvalho, Rui N. L. ;
Almeida, Rui M. ;
Moura, Jose J. G. ;
Lourenco, Nuno T. ;
Fonseca, Luis J. P. ;
Cordas, Cristina M. .
CHEMISTRYSELECT, 2016, 1 (20) :6546-6552
[37]   An amperometric biosensor based on the coimmobilization of horseradish peroxidase and methylene blue on a β-type zeolite modified electrode [J].
Baohong Liu ;
Zhenjiu Liu ;
Dandan Chen ;
Jilie Kong ;
Jiaqi Deng .
Fresenius' Journal of Analytical Chemistry, 2000, 367 :539-544
[38]   A novel one-dimensional chain-like structure based on sandwich-type tungstoantimonate and cadmium(II) [J].
Bi, Li-Hua ;
Li, Bao ;
Bo, Ya-Yan ;
Wu, Li-Xin .
INORGANICA CHIMICA ACTA, 2009, 362 (05) :1600-1604
[39]   BLOOD COAGULATION DYNAMICS: MATHEMATICAL MODELING AND STABILITY RESULTS [J].
Sequeira, Adelia ;
Santos, Rafael F. ;
Bodnar, Tomas .
MATHEMATICAL BIOSCIENCES AND ENGINEERING, 2011, 8 (02) :425-443
[40]   Mathematical and experimental modeling of reverse osmosis (RO) process [J].
Zeinab Hadadian ;
Sina Zahmatkesh ;
Mostafa Ansari ;
Ali Haghighi ;
Eskandar Moghimipour .
Korean Journal of Chemical Engineering, 2021, 38 :366-379