Involvement of transforming growth factor alpha (TGF alpha) and epidermal growth factor receptor (EGF(R)) in sex hormone-induced prostatic dysplasia and the growth of an androgen-independent transplantable carcinoma of the prostate

We previously reported the induction of dysplasia, a putative precursor of carcinoma, in the dorsolateral prostates (DLPs) of Noble rats by the combined administration of testosterone (T) and estradiol-17 beta (E(2)) for 16 weeks, Additionally, we demonstrated growth of the AIT, a DLP-derived, androgen-independent, transplantable solid tumor, in castrated syngeneic hosts. In this investigation, using Northern blot hybridization, radioimmunoassays and radioligand assays, we showed that transforming growth factor-alpha (TCF alpha) and epidermal growth factor receptor (EGF(R)) were expressed at close to non-detectable levels in the ventral prostates but at low, but measurable, levels in the DLPs of untreated rats, Enhanced expression of this ligand and its receptor was detected in the DLPs harboring dysplasia and marked overexpression of these molecules was noted in the AIT, In contrast, epidermal growth factor (EGF) expression was found to be constitutively expressed, at high levels, in both normal and dysplastic DLPs, but virtually absent in the AIT, Immunohistochemical data suggested that EGF, TGF alpha and EGF(R) were aprocine secretory products of the normal DLP, with TGF alpha and EGF localized to the supranuclear complexes and EGF(R) to the epical membranes of epithelial cells, Alterations in immunostaining patterns for TGF alpha and EGF(R) were exclusively detected in the dysplastic lesions in the DLPs of T + E(2)-treated rats, Enhanced intracytoplasmic localization for both peptides were found to accompany the loss of cell polarity in dysplastic foci, Strong intracytoplasmic immunostaining for TGF alpha was observed in some AIT cells whilst staining for EGF(R) was present in the membranes of tumor cells that formed psuedoacini, Taken together, our findings suggest that autocrine mechanisms may play an important role early in the carcinogenic process and that progression to an androgen-independent neoplastic growth may be modulated by this signaling pathway.