Live imaging of the Drosophila ovarian germline stem cell niche

被引:4
作者
Wilcockson, Scott G. [1 ,2 ]
Ashe, Hilary L. [1 ]
机构
[1] Univ Manchester, Fac Biol Med & Hlth, Manchester M13 9PT, England
[2] Francis Crick Inst, Dev Signalling Lab, 1 Midland Rd, London NW1 1AT, England
来源
STAR PROTOCOLS | 2021年 / 2卷 / 01期
基金
英国惠康基金;
关键词
Cell Biology; Microscopy; Model Organisms; Stem Cells;
D O I
10.1016/j.xpro.2021.100371
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
The maintenance of stem cell populations and the differentiation of their progeny is coordinated by specific communication with associated niche cells. Here, we describe a protocol for short-term live imaging of the Drosophila ovarian germline stem cell niche ex vivo. By immobilizing the ovarian tissue in a fibrinogen-thrombin clot, we are able to maintain the tissue for short-term high -temporal live imaging. This enables the visualization of dynamic cellular processes, such as the cytoskeletal dynamics that control stem cell niche communication. For complete details on the use and execution of this protocol, please refer to Wilcockson and Ashe (2019).
引用
收藏
页数:13
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