2-hydroxy-methyl-1(N-phtaloyltryptophyl) aziridine stimulates in vitro humain lymphocyte proliferation and interleukin secretion

被引:2
作者
Ahmed, E-Z. Baba [1 ]
Bouanane, S. [1 ]
Merzouk, S. -A. [2 ]
Merzouk, H. [1 ]
Medjahed, W.
Mulengi, J. Kajima
Prost, J. [3 ]
机构
[1] Univ Abou Bekr Belkaid, Lab Physiol Anim & Biochim, Dept Biol, Fac Sci, Imama 13000, Tlemcen, Algeria
[2] Univ Abou Bekr Belkaid, Dept Phys, Fac Sci, Imama 13000, Tlemcen, Algeria
[3] Univ Bourgogne, UFR Sci Vie, UPRES Lipides & Nutr 2422, F-21000 Dijon, France
来源
PATHOLOGIE BIOLOGIE | 2008年 / 56卷 / 03期
关键词
aziridines; interleukins; lymphocyte proliferation; mitogens;
D O I
10.1016/j.patbio.2007.05.004
中图分类号
R36 [病理学];
学科分类号
100104 ;
摘要
Aim of this work. - Aziridines have been shown to possess marked immunotropic activity. In this study, the in vitro effects of a new aziridine, 2-hydroxy-methyl-1-(N-phtaloyltryptophyl) aziridine, were determined on the proliferative responses of human lymphocytes stimulated by mitogens and on interleukin (IL-2, IL-6) secretion. Material and methods. - Peripheral blood lymphocytes were isolated using differential centrifugation on a density gradient of Ficoll-Paque. They were cultured with or without mitogens (Concanavalin A and lipopolysaccharide), and with different concentrations of the aziridine. Proliferation was monitored by direct cell counts and confirmed by MTT [3-(4,5-dimethyl thiazol-2-yl)-2,5-diphenyl tetrazolium bromide] assay. After different incubations, IL-2 and IL-6 were determined by using commercially avaible Elisa kits. Results. - The aziridine tested significantly stimulated the resting and mitogen T and B lymphocyte proliferation at concentrations between 1 mu M and 1 mM, in a dose-dependent manner. It also increased IL-2 and IL-6 secretion. Conclusion. - 2-hydroxy-methyi-1-(N-phtaloyltryptophyl) aziridine displayed immunomodulatory properties and is potentially immunostimulant. It could be used to provide non-specific cell-mediated immune responses. (C) 2007 Elsevier Masson SAS. Tons droits reserves.
引用
收藏
页码:137 / 142
页数:6
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