Label-free and sensitive thrombin sensing on a molecularly grafted aptamer on graphene

被引：53

作者：

Wang, Yanping ^{[1
]}

Xiao, Yinghong ^{[1
]}

Ma, Xiaoling ^{[2
]}

Lia, Na ^{[1
]}

Yang, Xiaodi ^{[1
]}

机构：

[1] Nanjing Normal Univ, Jiangsu Key Lab New Power Batteries, Jiangsu Key Lab Biofunct Mat, Lab Electrochem,Coll Chem & Mat Sci, Nanjing 210097, Peoples R China

[2] Nanjing Med Univ, Nanjing Matern & Child Hlth Care Hosp, Nanjing 210097, Peoples R China

来源：

CHEMICAL COMMUNICATIONS | 2012年 / 48卷 / 05期

关键词：

CARBON NANOTUBES; APTASENSOR; BIOSENSOR; PROTEINS; PLATFORM; OXIDE;

D O I：

10.1039/c1cc15429j

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A unique nanocomposite was constructed by grafting an aptamer on graphene and used as a label-free biosensor for thrombin sensing, showing good selectivity, a low detection limit of 0.45 fM and high sensitivity, with a linear response up to 100 fM.

引用

页码：738 / 740

页数：3

共 20 条

[11] Noncovalent DNA decorations of graphene oxide and reduced graphene oxide toward water-soluble metal-carbon hybrid nanostructures via self-assembly [J].

Liu, Jinbin ;

Li, Yulin ;

Li, Yueming ;

Li, Jinghong ;

Deng, Zhaoxiang .

JOURNAL OF MATERIALS CHEMISTRY, 2010, 20 (05) :900-906

[12] Aptamer conjugated Mo6S9-xIx nanowires for direct and highly sensitive electrochemical sensing of thrombin [J].

McMullan, Martin ;

Sun, Nijuan ;

Papakonstantinou, Pagona ;

Li, Meixian ;

Zhou, Wuzong ;

Mihailovic, Dragan .

BIOSENSORS & BIOELECTRONICS, 2011, 26 (05) :1853-1859

[13] Atomic Force and Scanning Tunneling Microscopy Imaging of Graphene Nanosheets Derived from Graphite Oxide [J].

Paredes, J. I. ;

Villar-Rodil, S. ;

Solis-Fernandez, P. ;

Martinez-Alonso, A. ;

Tascon, J. M. D. .

LANGMUIR, 2009, 25 (10) :5957-5968

[14] Single-walled carbon nanotube biosensors using aptamers as molecular recognition elements [J].

So, HM ;

Won, K ;

Kim, YH ;

Kim, BK ;

Ryu, BH ;

Na, PS ;

Kim, H ;

Lee, JO .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2005, 127 (34) :11906-11907

[15] Label-free electrochemical aptasensor for thrombin detection based on the nafion@graphene as platform [J].

Sun, Tao ;

Wang, Li ;

Li, Na ;

Gan, Xianxue .

BIOPROCESS AND BIOSYSTEMS ENGINEERING, 2011, 34 (09) :1081-1085

[16] Graphene and graphene oxide: biofunctionalization and applications in biotechnology [J].

Wang, Ying ;

Li, Zhaohui ;

Wang, Jun ;

Li, Jinghong ;

Lin, Yuehe .

TRENDS IN BIOTECHNOLOGY, 2011, 29 (05) :205-212

[17] Reusable electrochemical sensing platform for highly sensitive detection of small molecules based on structure-switching signaling aptamers [J].

Wu, Zai-Sheng ;

Guo, Meng-Meng ;

Zhang, Song-Bai ;

Chen, Chen-Rui ;

Jiang, Jian-Hui ;

Shen, Guo-Li ;

Yu, Ru-Qin .

ANALYTICAL CHEMISTRY, 2007, 79 (07) :2933-2939

[18] An aptamer-based biosensor for sensitive thrombin detection [J].

Yang, Hui ;

Ji, Ji ;

Liu, Yun ;

Kong, Jilie ;

Liu, Baohong .

ELECTROCHEMISTRY COMMUNICATIONS, 2009, 11 (01) :38-40

[19] A sensitive impedimetric thrombin aptasensor based on polyamidoamine dendrimer [J].

Zhang, Zhanxia ;

Yang, Wen ;

Wang, Juan ;

Yang, Cheng ;

Yang, Fan ;

Yang, Xiurong .

TALANTA, 2009, 78 (4-5) :1240-1245

[20] Electrochemical Sensing and Biosensing Platform Based on Chemically Reduced Graphene Oxide [J].

Zhou, Ming ;

Zhai, Yueming ;

Dong, Shaojun .

ANALYTICAL CHEMISTRY, 2009, 81 (14) :5603-5613

← 1 2 →