Label-free and sensitive thrombin sensing on a molecularly grafted aptamer on graphene

被引：53
作者：
Wang, Yanping [1 ]
Xiao, Yinghong [1 ]
Ma, Xiaoling [2 ]
Lia, Na [1 ]
Yang, Xiaodi [1 ]
机构：
[1] Nanjing Normal Univ, Jiangsu Key Lab New Power Batteries, Jiangsu Key Lab Biofunct Mat, Lab Electrochem,Coll Chem & Mat Sci, Nanjing 210097, Peoples R China
[2] Nanjing Med Univ, Nanjing Matern & Child Hlth Care Hosp, Nanjing 210097, Peoples R China
来源：
CHEMICAL COMMUNICATIONS | 2012年 / 48卷 / 05期
关键词：
CARBON NANOTUBES; APTASENSOR; BIOSENSOR; PROTEINS; PLATFORM; OXIDE;
D O I：
10.1039/c1cc15429j
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
A unique nanocomposite was constructed by grafting an aptamer on graphene and used as a label-free biosensor for thrombin sensing, showing good selectivity, a low detection limit of 0.45 fM and high sensitivity, with a linear response up to 100 fM.
引用
收藏
页码：738 / 740
页数：3
相关论文
共 20 条
[1]   Aptamer-based detection of plasma proteins by an electrochemical assay coupled to magnetic beads [J].
Centi, Sonia ;
Tombelli, Sara ;
Minunni, Maria ;
Mascini, Marco .
ANALYTICAL CHEMISTRY, 2007, 79 (04) :1466-1473
[2]   Graphene Fluorescence Resonance Energy Transfer Aptasensor for the Thrombin Detection [J].
Chang, Haixin ;
Tang, Longhua ;
Wang, Ying ;
Jiang, Jianhui ;
Li, Jinghong .
ANALYTICAL CHEMISTRY, 2010, 82 (06) :2341-2346
[3]   A new approach to fabricate graphene nanosheets in organic medium: combination of reduction and dispersion [J].
Che, Jianfei ;
Shen, Liying ;
Xiao, Yinghong .
JOURNAL OF MATERIALS CHEMISTRY, 2010, 20 (09) :1722-1727
[4]   Graphene-based materials in electrochemistry [J].
Chen, Da ;
Tang, Longhua ;
Li, Jinghong .
CHEMICAL SOCIETY REVIEWS, 2010, 39 (08) :3157-3180
[5]   Solid-contact potentiometric aptasensor based on aptamer functionalized carbon nanotubes for the direct determination of proteins [J].
Duezguen, Ali ;
Maroto, Alicia ;
Mairal, Teresa ;
O'Sullivan, Ciara ;
Rius, F. Xavier .
ANALYST, 2010, 135 (05) :1037-1041
[6]   In situ molecular detection of ischemic cells by enhanced protein direct electron transfer on a unique horseradish peroxidase-Au nanoparticles-polyaniline nanowires biofilm [J].
Guo, Chun Xian ;
Zheng, Xin Ting ;
Ng, Shu Rui ;
Lai, Yicheng ;
Lei, Yu ;
Li, Chang Ming .
CHEMICAL COMMUNICATIONS, 2011, 47 (09) :2652-2654
[7]   Materials science - Erasing electron mass [J].
Kane, CL .
NATURE, 2005, 438 (7065) :168-+
[8]   Aptamers based electrochemical biosensor for protein detection using carbon nanotubes platforms [J].
Kara, Pinar ;
de la Escosura-Muniz, Alfredo ;
Maltez-da Costa, Marisa ;
Guix, Maria ;
Ozsoz, Mehmet ;
Merkoci, Arben .
BIOSENSORS & BIOELECTRONICS, 2010, 26 (04) :1715-1718
[9]   Electrochemical impedance spectroscopy for study of aptamer-thrombin interfacial interactions [J].
Li, Xiaoxia ;
Shen, Lihua ;
Zhang, Dongdong ;
Qi, Honglan ;
Gao, Qiang ;
Ma, Fen ;
Zhang, Chengxiao .
BIOSENSORS & BIOELECTRONICS, 2008, 23 (11) :1624-1630
[10]   Electrochemical analysis of two analytes based on a dual-functional aptamer DNA sequence [J].
Li, Xuemei ;
Liu, Jinming ;
Zhang, Shusheng .
CHEMICAL COMMUNICATIONS, 2010, 46 (04) :595-597
12