Intramitochondrial accumulation of cationic Atto520-biotin proceeds via voltage-dependent slow permeation through lipid membrane

被引：3

作者：

Antonenko, Yuri N. ^{[1
]}

Nechaeva, Natalya L. ^{[2
]}

Baksheeva, Victoria E. ^{[2
]}

Rokitskaya, Tatyana I. ^{[1
]}

Plotnikov, Egor Y. ^{[1
]}

Kotova, Elena A. ^{[1
]}

Zorov, Dmitry B. ^{[1
]}

机构：

[1] Moscow MV Lomonosov State Univ, Belozersky Inst Phys Chem Biol, Moscow 119991, Russia

[2] Moscow MV Lomonosov State Univ, Dept Bioengn & Bioinformat, Moscow 119991, Russia

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES | 2015年 / 1848卷 / 06期

基金：

俄罗斯科学基金会;

关键词：

Rhodamine; Fluorescent dye; Penetrating cation; Mitochondria-targeted; Streptavidin; Fluorescence correlation spectroscopy; MITOCHONDRIA; STREPTAVIDIN; DERIVATIVES; BIOTIN; TRANSLOCATION; LOCALIZATION; ANTIOXIDANTS; PHLORETIN; TRANSPORT; BILAYERS;

D O I：

10.1016/j.bbamem.2015.02.028

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Conjugation to penetrating cations is a general approach for intramitochondrial delivery of physiologically active compounds, supported by a high membrane potential of mitochondria having negative sign on the matrix side. By using fluorescence correlation spectroscopy, we found here that Atto520-biotin, a conjugate of a fluorescent cationic rhodamine-based dye with the membrane-impermeable vitamin biotin, accumulated in energized mitochondria in contrast to biotin-rhodamine 110. The energy-dependent uptake of Atto520-biotin by mitochondria, being slower than that of the conventional mitochondrial dye tetramethyl-rhodamine ethyl ester, was enhanced by the hydrophobic anion tetraphenylborate (TPB). Atto520-biotin also exhibited accumulation in liposomes driven by membrane potential resulting from potassium ion gradient in the presence valinomycin. The induction of electrical current across planar bilayer lipid membrane by Atto520-biotin proved the ability of the compound to permeate through lipid membrane in a cationic form. Atto520-biotin stained mitochondria in a culture of L929 cells, and the staining was enhanced in the presence of TPB. Therefore, the fluorescent Atto520 moiety can serve as a vehicle for intramitochondrial delivery of hydrophilic drugs. Of importance for biotin-streptavidin technology, binding of Atto520-biotin to streptavidin was found to cause quenching of its fluorescence similar to the case of fluorescein-4-biotin. (C) 2015 Elsevier B.V. All rights reserved.

引用

页码：1277 / 1284

页数：8

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