Nanosecond time scale transient optoplasmonic detection of single proteins

被引：19

作者：

Baaske, Martin D. ^{[1
]}

Asgari, Nasrin ^{[1
]}

Punj, Deep ^{[1
]}

Orrit, Michel ^{[1
]}

机构：

[1] Leiden Univ, Huygens Kamerlingh Onnes Lab, Postbus 9504, NL-2300 RA Leiden, Netherlands

来源：

SCIENCE ADVANCES | 2022年 / 8卷 / 02期

关键词：

GLUCOSE-OXIDASE; DYNAMICS; COLLAPSE; NANORODS;

D O I：

10.1126/sciadv.abl5576

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Optical detection of individual proteins with high bandwidth holds great promise for understanding important biological processes on the nanoscale and for high-throughput fingerprinting applications. As fluorescent labels impose restrictions on detection bandwidth and require time-intensive and invasive processes, label-free optical techniques are highly desirable. Here, we read out changes in the resonantly scattered field of individual gold nanorods interferometrically and use photothermal spectroscopy to optimize the experiment's parameters. This interferometric plasmonic scattering enables the observation of single proteins as they traverse plasmonic near fields of gold nanorods with unprecedented temporal resolution in the nanosecond-to-microsecond range.

引用

页数：7

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