Sensitive Commercial NASBA Assay for the Detection of Respiratory Syncytial Virus in Clinical Specimen

被引：17
|
作者：
Tillmann, Ramona Liza [1 ]
Simon, Arne [2 ]
Mueller, Andreas [2 ]
Schildgen, Oliver [1 ]
机构：
[1] Univ Bonn, Inst Virol, D-5300 Bonn, Germany
[2] Univ Bonn, Childrens Hosp, D-5300 Bonn, Germany
来源：
PLOS ONE | 2007年 / 2卷 / 12期
关键词：
D O I：
10.1371/journal.pone.0001357
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
The aim of the study was to evaluate the usability of three diagnostic procedures for the detection of respiratory syncytial virus in clinical samples. Therefore, the FDA cleared CE marked NOW (R) RSV ELISA, the NucliSENS (R) EasyQ RSV A+B NASBA, and a literature based inhouse RT-PCR protocol were compared for their relative sensitivities. Thereby, NASBA turned out to be the most sensitive method with a total number of 80 RSV positive samples out of a cohort of 251 nasopharyngeal washings from patients suffering from clinical symptoms, followed by the inhouse RT-PCR (62/251) and ELISA (52/251). Thus, NASBA may serve as a rapid and highly sensitive alternative for RSV diagnostics.
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