Hepatocyte culture on 3D porous scaffolds of PCL/PMCL

被引：23

作者：

Xiao, Yan ^{[1
]}

Zhou, Miaomiao ^{[1
]}

Zhang, Mi ^{[2
]}

Liu, Wei ^{[2
]}

Zhou, Yan ^{[2
]}

Lang, Meidong ^{[1
]}

机构：

[1] East China Univ Sci & Technol, Sch Mat Sci & Engn, Key Lab Ultrafine Mat, Minist Educ, Shanghai 200237, Peoples R China

[2] East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

来源：

COLLOIDS AND SURFACES B-BIOINTERFACES | 2019年 / 173卷

基金：

中国国家自然科学基金; 上海市自然科学基金;

关键词：

Poly(epsilon-caprolactone); Porous scaffold; Crystalline degree; Hepatocyte culture; EXTRACELLULAR-MATRIX; MECHANICAL-PROPERTIES; IN-VITRO; PCL/PLCL BLEND; CELL-CULTURE; HYDROGELS; GROWTH; BIOMATERIALS; FIBROBLASTS; STIFFNESS;

D O I：

10.1016/j.colsurfb.2018.09.064

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

The development of three-dimensional (3D) porous scaffolds for soft tissue engineering mainly focused on manipulation of scaffold properties with cell behaviors. By emulsion freeze-drying method, four types of porous scaffolds were prepared from amorphous poly(4-methy-epsilon-caprolactone) (PMCL) and semi-crystalline poly(epsilon-caprolactone) (PCL) at different weight ratios, named as PMCLO, PMCL30, PMCL50 and PMCL70, respectively. Visual observation on cross-sectional images of the scaffolds appeared as sponge-like materials with three-dimensional and highly porous morphologies. However, the pore size, porosity and wettability of blends were not decreased linearly with increasing amorphous PMCL. Distinguished from PMCL30 or PMCL70, PMCL50 preserved intact PCL crystals distributed in amorphous matrix, resulting in the lowest Young's modulus (E) and relatively high wettability. From in vitro cell culture, it was observed that PMCL50 scaffold supported human induced hepatocytes (hiHeps) proliferation and function preservation best among all scaffolds. hiHeps on PMCL50 inclined to adopt fibroblastic morphology, whereas formed spheroidal morphology on PMCLO. It was suggested that our bare scaffolds with tailored properties have shown remarkable capability towards hiHep proliferation and function expression.

引用

页码：185 / 193

页数：9

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