An algal lectin griffithsin inhibits Hantaan virus infection in vitro and in vivo

被引：10

作者：

Zhao, Yajing ^{[1
,2
]}

Zhao, Ningbo ^{[1
,2
]}

Cai, Yanxing ^{[3
,4
]}

Zhang, Hui ^{[2
]}

Li, Jia ^{[5
]}

Liu, Jiaqi ^{[1
]}

Ye, Chuantao ^{[6
]}

Wang, Yuan ^{[2
]}

Dang, Yamei ^{[2
]}

Li, Wanying ^{[2
,7
]}

Liu, He ^{[2
]}

Zhang, Lianqing ^{[1
]}

Li, Yuexiang ^{[1
]}

Zhang, Liang ^{[2
]}

Cheng, Linfeng ^{[2
]}

Dong, Yangchao ^{[2
]}

Xu, Zhikai ^{[2
]}

Lei, Yingfeng ^{[2
]}

Lu, Lu ^{[4
]}

Wang, Yingjuan ^{[1
]}

Ye, Wei ^{[2
]}

Zhang, Fanglin ^{[2
]}

机构：

[1] Northwest Univ, Coll Life Sci, Xian, Shaanxi, Peoples R China

[2] Airforce Med Univ, Sch Preclin Med, Dept Microbiol, Xian, Shaanxi, Peoples R China

[3] Guiyang Maternal & Child Hlth Care Hosp, Guiyang, Guizhou, Peoples R China

[4] Fudan Univ, Sch Basic Med Sci, Key Lab Med Mol Virol MOE NHC CAMS, BSL 3 Facil, Shanghai, Peoples R China

[5] Xian Int Med Ctr Hosp, Dept Neurol, Xian, Shaanxi, Peoples R China

[6] Airforce Med Univ, Tangdu Hosp, Dept Infect Dis, Xian, Shaanxi, Peoples R China

[7] Yanan Univ, Sch Preclin Med, Dept Pathogen Biol, Yanan, Shaanxi, Peoples R China

来源：

FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY | 2022年 / 12卷

基金：

中国国家自然科学基金;

关键词：

griffithsin (GRFT); hantavirus; Hantaan virus; hemorrhagic fever with renal syndrome (HFRS); lectin; antivirals; suckling mice; vesicular stomatitis virus (VSV); PROTEIN; FUSION;

D O I：

10.3389/fcimb.2022.881083

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Hantaan virus (HTNV) is the etiological pathogen of hemorrhagic fever with renal syndrome in East Asia. There are currently no effective therapeutics approved for HTNV and other hantavirus infections. We found that griffithsin (GRFT), an algae-derived lectin with broad-spectrum antiviral activity against various enveloped viruses, can inhibit the growth and spread of HTNV. In vitro experiments using recombinant vesicular stomatitis virus (rVSV) with HTNV glycoproteins as a model revealed that the GRFT inhibited the entry of rVSV-HTNV-G into host cells. In addition, we demonstrated that GRFT prevented authentic HTNV infection in vitro by binding to the viral N-glycans. In vivo experiments showed that GRFT partially protected the suckling mice from death induced by intracranial exposure to HTNV. These results demonstrated that GRFT can be a promising agent for inhibiting HTNV infection.

引用

页数：14

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