New primers reveal the presence of a duplicate histone H3 in the marine turtle leech Ozobranchus branchiatus

被引：0

作者：

Lavretsky, Philip ^{[1
]}

Truong, Triet M. ^{[2
]}

McGowin, Audrey E. ^{[2
]}

Balazs, George H. ^{[3
]}

Peters, Jeffrey L. ^{[1
]}

机构：

[1] Wright State Univ, Dept Biol Sci, Dayton, OH 45435 USA

[2] Wright State Univ, Dept Chem, Dayton, OH 45435 USA

[3] NOAA, Natl Marine Fisheries Serv, SW Fisheries Sci Ctr, Honolulu Lab, Honolulu, HI 96822 USA

来源：

CONSERVATION GENETICS RESOURCES | 2012年 / 4卷 / 02期

关键词：

Ozobranchus branchiatus; Fibropapillomatosis; Histone H3; Primers; Gene duplication; Chelonia mydas; PHYLOGENY; VARIANTS; MOLLUSCA; VECTOR; GENES;

D O I：

10.1007/s12686-011-9581-6

中图分类号：

X176 [生物多样性保护];

学科分类号：

090705 ;

摘要：

Marine leeches, specific to sea turtles, have been implicated as potential vector organisms in the spread of fibropapillomatosis (FP), a pandemic neoplastic disease with Chelonia mydas having the highest affliction rate. Polymerase chain reaction identified two independent, seemingly functional histone H3 loci for marine turtle leeches Ozobranchus branchiatus collected from C. mydas in Florida and Hawaii. Primers were developed to amplify each product separately. Among the two loci, sequence differentiation (I broken vertical bar (ST) ) ranged from 0.161 to 0.182 with identical amino acid translations among the 22 samples. A maximum parsimony tree of GenBank histone H3 sequences from annelids indicated the gene duplication occurred within the Ozobranchidae family. Geographically separated populations yielded I broken vertical bar (ST) values of 0.004-0.005 but were phylogenetically distinctive. These novel markers will be useful in identifying ectoparasites in FP research, evaluating other histone variants, and chromatin dynamics regulation studies.

引用

页码：487 / 490

页数：4

共 19 条

[1] Recognition and classification of histones using support vector machine [J].