Single cell transcriptomics of neighboring hyphae of Aspergillus niger

被引：60

作者：

de Bekker, Charissa ^{[1
]}

Bruning, Oskar ^{[2
,3
]}

Jonker, Martijs J. ^{[2
,3
,4
]}

Breit, Timo M. ^{[2
,3
,4
]}

Wosten, Han A. B. ^{[1
]}

机构：

[1] Univ Utrecht, Inst Biomembranes, Kluyver Ctr Genom Ind Fermentat, NL-3584 CH Utrecht, Netherlands

[2] Univ Amsterdam, Swammerdam Inst Life Sci, Microarray Dept, NL-1098 XH Amsterdam, Netherlands

[3] Univ Amsterdam, Swammerdam Inst Life Sci, Integrat Bioinformat Unit, NL-1098 XH Amsterdam, Netherlands

[4] NBIC, NL-6525 GA Nijmegen, Netherlands

来源：

GENOME BIOLOGY | 2011年 / 12卷 / 08期

关键词：

GENE-EXPRESSION; NEUROSPORA-CRASSA; RNA-SYNTHESIS; PROTEINS; GROWTH; LOCALIZATION; SECRETION; DIFFERENTIATION; TRANSFORMATION; HYBRIDIZATION;

D O I：

10.1186/gb-2011-12-8-r71

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Single cell profiling was performed to assess differences in RNA accumulation in neighboring hyphae of the fungus Aspergillus niger. A protocol was developed to isolate and amplify RNA from single hyphae or parts thereof. Microarray analysis resulted in a present call for 4 to 7% of the A. niger genes, of which 12% showed heterogeneous RNA levels. These genes belonged to a wide range of gene categories.

引用

页数：12

共 43 条

[1]

Alberts B., 1994, MOL BIOL CELL

[2]

[Anonymous], [No title captured]

[3]

[Anonymous], THESIS U UTRECHT

[4]

[Anonymous], CURR BIOL

[5] Evaluation of methods for amplification of picogram amounts of total RNA for whole genome expression profiling [J].

Clement-Ziza, Mathieu ;

Gentien, David ;

Lyonnet, Stanislas ;

Thiery, Jean-Paul ;

Besmond, Claude ;

Decraene, Charles .

BMC GENOMICS, 2009, 10

[6] An enzyme cocktail for efficient protoplast formation in Aspergillus niger [J].

de Bekker, Charissa ;

Wiebenga, Ad ;

Aguilar, Guillermo ;

Wosten, Han A. B. .

JOURNAL OF MICROBIOLOGICAL METHODS, 2009, 76 (03) :305-306

[7] Heterogeneity of Aspergillus niger Microcolonies in Liquid Shaken Cultures [J].

de Bekker, Charissa ;

van Veluw, G. Jerre ;

Vinck, Arman ;

Wiebenga, L. Ad ;

Wosten, Han A. B. .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2011, 77 (04) :1263-1267

[8] Partial nuclear pore complex disassembly during closed mitosis in Aspergillus nidulans [J].

De Souza, CPC ;

Osmani, AH ;

Hashmi, SB ;

Osmani, S .

CURRENT BIOLOGY, 2004, 14 (22) :1973-1984

[9] A new black Aspergillus species, A. vadensis, is a promising host for homologous and heterologous protein production [J].

de Vries, RP ;

Burgers, K ;

van de Vondervoort, PJI ;

Frisvad, JC ;

Samson, RA ;

Visser, J .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2004, 70 (07) :3954-3959

[10] Regulation of the α-glucuronidase-encoding gene (aguA) from Aspergillus niger [J].

de Vries, RP ;

van de Vondervoort, PJI ;

Hendriks, L ;

van de Belt, M ;

Visser, J .

MOLECULAR GENETICS AND GENOMICS, 2002, 268 (01) :96-102

← 1 2 3 4 5 →